Template:SUIT-010

Steps and respiratory states

Step	State	Pathway	Q-junction	Comment - Events (E) and Marks (M)
ce2Rot	ROX		CII	ce1;ce2Rot Succinate pathway control state (S-pathway) after inhibiting CI with rotenone, which also inhibits the F-pathway.
ce3S	SL	S	CII	ce1;ce2Rot;ce3S Succinate, S ( type S-pathway to Q). Template:SUIT L n
ce4D	SP	S	CII	ce1;ce2Rot;ce3S;ce4D Succinate, S ( type S-pathway to Q). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
1Dig	SP	S	CII	ce1;ce2Rot;ce3S;ce4D;1Dig Succinate, S ( type S-pathway to Q). Template:SUIT Digitonin titration OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.
1c	SP	S	CII	ce1;ce2Rot;ce3S;ce4D;1Dig;1c Succinate, S ( type S-pathway to Q). Addition of cytochrome c yields a test for integrity of the mtOM (cytochrome c control efficiency). Stimulation by added cytochrome c would indicate an injury of the mtOM and limitation of respiration in the preceding state without added c due to loss of cytochrome c. Typically, cytochrome c is added immediately after the earliest ADP-activation step (OXPHOS capacity P with saturating [ADP]). OXPHOS capacity P (with saturating [ADP]), active OXPHOS state.