Template:SUIT text D009: Difference between revisions
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The SUIT-003 O2 ce D009 (CCP-ce) is designed to study [[coupling control state |coupling control]] of living cells. Respiratory capacities are tested in a sequence of coupling states: [[ROUTINE]], [[LEAK]] and [[ET]]. To study [[LEAK-respiration]], the [[phosphorylation system]] is inhibited by [[oligomycin]]. The final concentration of [[oligomycin]] has to be carefully optimized for various cell types, to minimize the inhibitory effect on the [[Electron transfer-pathway|electron transfer system]] which would lead to an underestimation of [[ET-capacity]]. | |||
Revision as of 14:04, 23 July 2019
The SUIT-003 O2 ce D009 (CCP-ce) is designed to study coupling control of living cells. Respiratory capacities are tested in a sequence of coupling states: ROUTINE, LEAK and ET. To study LEAK-respiration, the phosphorylation system is inhibited by oligomycin. The final concentration of oligomycin has to be carefully optimized for various cell types, to minimize the inhibitory effect on the electron transfer system which would lead to an underestimation of ET-capacity.
