Template:SUIT text D027
From Bioblast
The SUIT-012 O2 mt D027 protocols specifically focus on assessing the linear coupling control (L- P- E) with NADH-linked substrates (PM) in mitochondrial preparations such as isolated mitochondria, tissue homogenates and permeabilized cells (already permeabilized when they are added to the chamber) in a wide variety of species and tissues. Addition of G in NADH-supported OXPHOS enables evaluating the glutamate anaplerotic pathway control state. SUIT-012 O2 mt D027 can be extended with the CIV assay module.
