Template:SUIT text D028: Difference between revisions
From Bioblast
(Created page with "SUIT-003 O2 ce D028 is a coupling control protocol for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use o...") |
Beno Marija (talk | contribs) No edit summary |
||
| Line 1: | Line 1: | ||
SUIT-003 O2 ce D028 is a [[coupling control protocol]] for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use of [[oligomycin]] provides important information about the ETS and TCA activity but the optimal concentration has to be carefully determined experimentally to avoid the well-known side effects over the ET-capacity. After the addition of [[Rotenone]] to reach ROX state we add succinate, which does not permeate the plasma membrane, to test if the plasma membrane of the cells is altered (permeability test). If we do not obtain further stimulation of the respiration we could conclude that the living cells are not permeable and remain intact. | SUIT-003 O2 ce D028 is a [[coupling-control protocol]] for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use of [[oligomycin]] provides important information about the ETS and TCA activity but the optimal concentration has to be carefully determined experimentally to avoid the well-known side effects over the ET-capacity. After the addition of [[Rotenone]] to reach ROX state we add succinate, which does not permeate the plasma membrane, to test if the plasma membrane of the cells is altered (permeability test). If we do not obtain further stimulation of the respiration we could conclude that the living cells are not permeable and remain intact. | ||
Latest revision as of 16:29, 2 June 2020
SUIT-003 O2 ce D028 is a coupling-control protocol for living cells. Different coupling control states are assessed (ROUTINE - L(Omy) - E) at ce-substrate state. The use of oligomycin provides important information about the ETS and TCA activity but the optimal concentration has to be carefully determined experimentally to avoid the well-known side effects over the ET-capacity. After the addition of Rotenone to reach ROX state we add succinate, which does not permeate the plasma membrane, to test if the plasma membrane of the cells is altered (permeability test). If we do not obtain further stimulation of the respiration we could conclude that the living cells are not permeable and remain intact.
