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Template talk:Next oroboros ecosystem agenda

From Bioblast
Revision as of 09:19, 22 July 2020 by Antunes Diana (talk | contribs)
Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
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e.g.,

O2k-Open SupportWarning: loss of data Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?

- »Instrumental background« and »TIP2k Filter Papers«

See the scheme:


Agenda/Alerts body text format


Bioblast alert 2020

Prep for circular done here: Talk:Bioblast alert 2020

MitoPedia alert

Next

  • Aug-06 Th

O2k-Brief alert

Next

O2k-Publications alert

Next

  • Jul-28 Tu
O2k-brief repository Insights into Hepatitis C virus-linked mitochondrial dysfunction and the potential development of hepatitis C-linked carcinogenic transformation
from the O2k-Network »IT Foggia Capitanio N« 
  • Ripoli M, D'Aprile A, Quarato G, Sarasin-Filipowicz M, Gouttenoire J, Scrima R, Cela O, Boffoli D, Heim MH, Moradpour D, Capitanio N, Piccoli C (2009) Hepatitis C virus-linked mitochondrial dysfunction promotes hypoxia-inducible factor 1α-mediated glycolytic adaptation. J Virol 84:647-60. »Bioblast link«
  • Aug-04 Tu
O2k-Publications in the MiPMap Deletion of Prkn impairs mitochondrial respiratory capacity: a non-alcoholic fatty liver disease pathogenesis
from the O2k-Network »US PA Pittsburgh Jurczak MJ«
  • Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. »Bioblast link«

O2k-Open Support alert

Next

  • While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.


  • Jul-27 Mo

Option 1:

O2k-Open SupportWarning: loss of data Do you know how to prepare a homogenate from animal tissue using the PBI-Shredder O2k-Set?

See how to use this auxiliary O2k-Tool and obtain well-coupled mitochondria preparation.
- » O2k-Videosupport for shredder tissue homogenate«

Option 2:

O2k-Open SupportInfo: improve Do you know how to use the PBI-Shredder O2k-Set to obtain well-coupled mitochondria preparation?

- » O2k-Videosupport for shredder tissue homogenate« and » O2k-Videosupport for O2k-Mitochondrial preparations«

Option 3:

O2k-Open SupportWarning: loss of data Reproducibility issues with mitochondrial preparations are often caused by variations during the isolation method.

The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility of mitochondrial yield and mitochondrial function.
- » O2k-Videosupport for shredder tissue homogenate«

Option 4:

O2k-Open SupportInfo: improve Did you know that Reproducibility issues with mitochondrial preparations caused by variations during the isolation method can be assisted by the PBI-Shredder O2k-Set?

The PBI-Shredder O2k-Set provides a standardized approach to prepare homogenates of various tissues with high reproducibility of mitochondrial yield and mitochondrial function.
- » O2k-Videosupport for shredder tissue homogenate«


  • Aug-10 Mo

Removed for correction

O2k-Open SupportWarning: loss of data Do you perform the leaky chamber test after chamber assembly?

An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- »Leaky chamber test« and » https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assembly«

O2k-Open Support alert circulars

O2k-Open Support alert circular 2020(01): in prep

New O2k-Open SupportWarning: loss of data A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.

See how you can check that the two blue OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«

New O2k-Open SupportTake care Calibration parameters are transferred to experimental DatLab files

Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files.
- » Oxygen calibration - DatLab«

O2k-Open SupportWarning: loss of data Do you perform O2k-Chamber volume calibration?

The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module).
- »O2k-Chamber MitoPedia«

O2k-Open SupportTake care Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?

The O2 solubility factor varies according to the medium used and influences the air calibration.
- »Oxygen solubility factor«

In preparation

For discussion

Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
MitoPedia: SUIT Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions:
  • Proven -80 °C solid stability for more than 3 years (3 years 7 months)
  • Proven -20 °C solid stability for 1 year
  • Proven DMSO stability at room temperature for 1 week
  • Proven DMSO stability at -20 °C for 6 months (ongoing)

2021

Postponed events

FAT4BRAIN » FAT4BRAIN School Riga LV IOC147«
»APS2020 Chicago US«
O2k-Workshops San Diego CA US