Vásquez-Vivar 1997 FEBS Letters
| Vásquez-Vivar J, Hogg N, Pritchard Jr KA, Martasek P, Kalyanaraman B (1997) Superoxide anion formation from lucigenin: an electron spin resonance spin-trapping study. FEBS Letters 403:127-30. |
Vásquez-Vivar J, Hogg N, Pritchard Jr KA, Martasek P, Kalyanaraman B (1997) FEBS Letters
Abstract: Lucigenin (LC2+) is frequently used as a superoxide probe. To detect superoxide, lucigenin must be reduced to the lucigenin cation radical (LC.+). We show, using the phosphorylated spin trap 5-diethoxyphosphoryl-5-methyl-1-pyrroline N-oxide (DEPMPO), that lucigenin stimulates NADPH-dependent superoxide production by endothelial nitric oxide synthase (eNOS). The formation of the DEPMPO-superoxide adduct is calcium/calmodulin independent. DEPMPO-superoxide adduct formation is inhibited by diphenyleneiodonium and is abolished by superoxide dismutase. It is likely that eNOS/NADPH can reduce lucigenin to LC.+ which reduces oxygen to superoxide. Consequently, lucigenin cannot be used to measure superoxide formation.
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