Difference between revisions of "Ye 2011 J Biol Chem"
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{{Publication | {{Publication | ||
|title=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) | |title=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor Ī³ (PPARĪ³) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286:40013-24. | ||
|info=[http://www.ncbi.nlm.nih.gov/pubmed/21917928 PMID:21917928 ] | |info=[http://www.ncbi.nlm.nih.gov/pubmed/21917928 PMID:21917928 Open Access] | ||
|authors=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E | |authors=Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E | ||
|year=2011 | |year=2011 | ||
|journal=J | |journal=J Biol Chem | ||
|abstract=Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical Warburg effect. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism | |abstract=Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical [[Warburg effect]]. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARĪ³ and co-activates PPARĪ³-driven transcription. | ||
|keywords=Chicken embryo fibroblasts (CEFs), | |||
|mipnetlab=US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H | |||
}} | }} | ||
{{Labeling | {{Labeling | ||
|area=Respiration, mt-Biogenesis;mt-density | |||
|organism=Chicken | |||
|tissues=Fibroblast | |||
|preparations=Intact cells | |||
|topics=Aerobic glycolysis | |||
|couplingstates=ROUTINE | |||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
}} | }} |
Latest revision as of 15:47, 9 November 2016
Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) Peroxisome proliferator-activated receptor Ī³ (PPARĪ³) mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286:40013-24. |
Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) J Biol Chem
Abstract: Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical Warburg effect. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through Ī²-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARĪ³, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARĪ³ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARĪ³ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARĪ³ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARĪ³ and co-activates PPARĪ³-driven transcription. ā¢ Keywords: Chicken embryo fibroblasts (CEFs)
ā¢ O2k-Network Lab: US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H
Labels: MiParea: Respiration, mt-Biogenesis;mt-density
Organism: Chicken
Tissue;cell: Fibroblast
Preparation: Intact cells
Regulation: Aerobic glycolysis Coupling state: ROUTINE
HRR: Oxygraph-2k