Difference between revisions of "Ye 2011 J Biol Chem"
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|area=Respiration, mt-Biogenesis; mt-density | |area=Respiration, mt-Biogenesis;mt-density | ||
|organism=Chicken | |organism=Chicken | ||
|model cell lines=Fibroblast | |model cell lines=Fibroblast |
Revision as of 19:07, 11 August 2013
Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) PPAR{gamma} mediates a Ski-induced shift from glycolysis to oxidative energy metabolism. J Biol Chem 286: 40013-40024. |
Ye F, Lemieux H, Hoppel CL, Hanson RW, Hakimi P, Croniger CM, Puchowicz M, Anderson VE, Fujioka H, Stavnezer E (2011) J Biol Chem
Abstract: Over-expression of the Ski oncogene induces oncogenic transformation of chicken embryo fibroblasts (CEFs). However, unlike most other oncogene-transformed cells, Ski-transformed CEFs (Ski-CEFs) do not display the classical Warburg effect. On the contrary, Ski transformation reduced lactate production and glucose utilization in CEFs. Compared to CEFs, Ski-CEFs exhibited enhanced TCA cycle activity, fatty acid catabolism through β-oxidation, glutamate oxidation, oxygen consumption, as well as, increased numbers and mass of mitochondria. Interestingly, expression of PPARγ, a key transcription factor that regulates adipogenesis and lipid metabolism was dramatically elevated at both the mRNA and protein levels in Ski-CEFs. Accordingly, PPARγ target genes that are involved in lipid uptake, transport and oxidation were also markedly up-regulated by Ski. Knocking down PPARγ in Ski-CEFs by RNA interference reversed the elevated expression of these PPARγ target genes, as well as the shift to oxidative metabolism and the increased mitochondrial biogenesis. Moreover, we found that Ski co-immunoprecipitates with PPARγ and co-activates PPARγ-driven transcription. • Keywords: Chicken embryo fibroblasts (CEFs)
• O2k-Network Lab: US_OH_Cleveland_Hoppel_CL, CA_Edmonton_Lemieux_H
Labels: MiParea: Respiration, mt-Biogenesis;mt-density
Organism: Chicken
Preparation: Intact cells
Regulation: Aerobic glycolysis Coupling state: ROUTINE
HRR: Oxygraph-2k