SUIT-008 O2 pfi D014: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=NS(PGM) | |||
|description=[[File:1PM;2D;3G;4S;5U;6Rot-.png|300px]] | |description=[[File:1PM;2D;3G;4S;5U;6Rot-.png|300px]] | ||
|info='''A: [[SUIT-008]] protocol in [[MitoEAGLE]] project (WG2) for testing the skills preparing permeabilized fibers''' [[Lemieux 2017 Sci Rep]] | |info='''A: [[SUIT-008]] protocol in [[MitoEAGLE]] project (WG2) for testing the skills preparing permeabilized fibers''' [[Lemieux 2017 Sci Rep]] | ||
|application=O2 | |SUIT number=D14_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd | ||
|application=O2 | |||
}} | |||
{{MitoPedia concepts | |||
|mitopedia concept=SUIT protocol | |||
}} | }} | ||
{{MitoPedia methods | |||
|mitopedia method=Respirometry | |||
}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
{{MitoPedia topics}} | |||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' NS(PGM) | ||
::: '''[[SUIT protocol pattern]]:''' diametral 1PM;2D;3G;4S;5U;6Rot | ::: '''[[SUIT protocol pattern]]:''' diametral 1PM;2D;3G;4S;5U;6Rot | ||
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|sort=Was published in year | |sort=Was published in year | ||
|order=descending | |order=descending | ||
}} | }} |
Revision as of 14:25, 15 January 2019
Description
Abbreviation: NS(PGM)
Reference: A: SUIT-008 protocol in MitoEAGLE project (WG2) for testing the skills preparing permeabilized fibers Lemieux 2017 Sci Rep
SUIT number: D14_1PM;2D;2c;3G;4S;4D;5U;6Rot;7Ama;8AsTm;9Azd
O2k-Application: O2
MitoPedia concepts:
SUIT protocol
MitoPedia methods:
Respirometry
- SUIT-category: NS(PGM)
- SUIT protocol pattern: diametral 1PM;2D;3G;4S;5U;6Rot
1PM;2D;2c;3G;4S;5U;6Rot-
Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
---|---|---|---|---|
1PM | PM(L) | N | CI | |
2D | PM(P) | N | CI | |
2D(2c) | PM(c) | N | CI | |
3G | PGM(P) | N | CI | |
4S | PGMS(P) | NS | CI&II | |
5U | PGMS(E) | NS | CI&II | |
6Rot | S(E) | S | CII | |
7Ama | ROX |
1PM;2D;3G;3c;4S;5U;6Rot-
Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
---|---|---|---|---|
1PM | PM(L) | N | CI | |
2D | PM(P) | N | CI | |
3G | PGM(P) | N | CI | |
G(c) | PGM(c) | N | CI | Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-. |
4S | PGMS(P) | NS | CI&II | |
5U | PGMS(E) | NS | CI&II | |
6Rot | S(E) | S | CII | |
7Ama | ROX |
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 | Mouse | Heart |