SUIT-016: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=FNS(Oct,GM) | |abbr=FNS(Oct,GM) | ||
|description=[[File:1OctM;2D;3G;4S;5Rot;6Omy;7U-.png| | |description=[[File:1OctM;2D;3G;4S;5Rot;6Omy;7U-.png|400px]] | ||
|info='''A:[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] in [[LEAK-respiration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]]''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-016.pdf|Bioblast pdf]] Β»[http://wiki.oroboros.at/index.php/File:SUIT-016.pdf Versions] | |info='''A:[[Fatty_acid_oxidation_pathway_control_state|F-pathway]] in [[LEAK-respiration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]]''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-016.pdf|Bioblast pdf]] Β»[http://wiki.oroboros.at/index.php/File:SUIT-016.pdf Versions] | ||
}} | }} |
Revision as of 14:59, 21 February 2019
Description
Abbreviation: FNS(Oct,GM)
Reference: A:F-pathway in LEAK state and OXPHOS state Β»Versions
- SUIT-category: FNS(Oct,GM)
- SUIT protocol pattern: diametral/complex 1OctM;2D;3G;4S;5Rot;6Omy;7U-
SUIT-016 gives information on F-pathway in LEAK state and OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. In addition, the pathway control of FN and FNS in OXPHOS state and of S in LEAK state and ET state is evaluated.
Communicated by Doerrier C, Huete-Ortega M and Gnaiger E (last update 2019-01-30)
Specific SUIT protocols
- SUIT-016 O2 pfi D044 for permeabilized fibers
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1OctM | OctML(n) | F(N) | CETF | 1OctM
|
2D | OctMP | F(N) | CETF | 1OctM;2D
|
3G | OctGMP | FN | CETF&I | 1OctM;2D;3G
|
4S | OctGMSP | FNS | CETF&CI&II | 1OctM;2D;3G;4S
|
5Rot | SP | S | CII | 1OctM;2D;3G;4S;5Rot
|
6Omy | SL(Omy) | S | CII | 1OctM;2D;3G;4S;5Rot;6Omy
|
7U | SE | S | CII | 1OctM;2D;3G;4S;5Rot;6Omy;7U
|
8Ama | ROX | 1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- + This protocol provides information on FAO capacity in the absence of other potentially interfering pathways both in LEAK and OXPHOS coupling control states.
- + FNS OXPHOS capacity comprises the most important pathways in many cell types and thus provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity.
- + FNS ET capacity is a good estimate of overall ET capacity in many cell types.
- + This is a good protocol to analyse coupling control at S-pathway, which is an advantage compared to SUIT-015 and SUIT-017.
- + Glutamate is easier to prepare compared to pyruvate.
- - In some tissues GM is not sufficient to fully support N-pathway capacity
- - SRot(E) may be underestimated if S is not saturating.
- - SRot(E) may be underestimated in the presence of Omy in some tissues and cell types
- - CIV activity is not measured, to save experimental time.
- It is possible to add cytochrome c in different steps:
- (2c), when cytochrome c is added after 2D, it allows to compare all steps in OXPHOS even if there is damage of the mitochondrial outer membrane, and can be chosen when the cytochrome c effect is not an exclusion criteria.
- (3c) when cytochrome c is added after 3G, due to the higher flux, it is easier to notice whether there is an increase in respiration after addition of cytochrome c. However, if there is a strong cytochrome c effect, it is not possible to compare OctMP with the next steps in OXPHOS.
- (7c) when cytochrome c is added after 7U, can be used as an exclusion criteria.
Compare SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Gnaiger 2015 Scand J Med Sci Sports | 2015 | Gnaiger E, Boushel R, SΓΈndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DΓez-SΓ‘nchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 | Human | Skeletal muscle |
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry