Template:SUIT-003: Difference between revisions
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[[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | [[Image:SUIT-MitoFit.png|right|190px|link=http://www.bioblast.at/index.php/MitoPedia:_SUIT |MitoPedia: SUIT]] | ||
== Steps and respiratory states == | == Steps and respiratory states == | ||
[[File: | [[File:Ce1;ce2(Omy);ce3U-.png|250px]] | ||
{| class="wikitable" border="1" | {| class="wikitable" border="1" | ||
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|- | |- | ||
| | | ce1 | ||
| [[ | | [[ROUTINE]] | ||
| | |||
| Β | | Β | ||
| ce1 | |||
| ce1 | {{Template:SUIT ROUTINE}} | ||
Β | |||
{{Template:SUIT | |||
|- | |- | ||
| | | ce2Omy | ||
| [[ | | ''[[LEAK respiration|L]]'' | ||
| | | Β | ||
| | | Β | ||
| ce1; | | ce1;ce2Omy | ||
{{Template:SUIT L(Omy)}} Β | |||
*The addition of [[oligomycin]] or its carrier is optional depending on the experimental question to resolve. | |||
|- | |- | ||
| | | ce3U | ||
| [[ | | ''[[E]]'' | ||
| | | Β | ||
| | | | ||
| ce1; | | ce1;ce2Omy;ce3U | ||
{{Template:SUIT | {{Template:SUIT U*}} {{Template:SUIT ET}} | ||
|- | |- | ||
| | | ce4Rot | ||
| | | ''[[ROX]]'' | ||
| Β | |||
| | |||
| ce1;ce2Omy;ce3U;ce4Rot | |||
Β | |||
Β | |||
| | |||
| | |||
| ce1; | |||
{{Template:SUIT | {{Template:SUIT ROX}} | ||
|} | |} | ||
Latest revision as of 13:07, 25 November 2020
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
ROUTINE respiration in the physiological coupling state R. Externally added permeable substrates could contribute to this respiratory state. | ||
ce2Omy | L | ce1;ce2Omy
Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
| ||
ce3U | E | ce1;ce2Omy;ce3U
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. Noncoupled electron transfer state, ET state, with ET capacity E. | ||
ce4Rot | ROX | ce1;ce2Omy;ce3U;ce4Rot
Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated either after inhibition of CIII (e.g. antimycin A, myxothiazol), CIV (e.g. Cyanide) or in the absence of endogenous fuel-substrates. Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration. |