Moreno-Sanchez 2022 Abstract Bioblast: Difference between revisions
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[[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | [[File:Bioblast2022 banner.jpg|link=Bioblast_2022]] | ||
{{Abstract | {{Abstract | ||
|title=<u>Moreno- | |title=6.2. '''«10+5»''' [[File:Profile-icon-9.png|left|100px]] <u>Moreno-Sanchez Rafael</u>, Robledo-Cadena DX, Pacheco-VelĂĄzquez SC, RodrĂguez-EnrĂquez S (2022) Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect. '''Bioblast 2022: BEC Inaugural Conference.''' In: https://doi.org/10.26124/bec:2022-0001 | ||
|info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | |info=[https://wiki.oroboros.at/index.php/Bioblast_2022#Submitted_abstracts Bioblast 2022: BEC Inaugural Conference] | ||
|authors= Moreno-Sanchez Rafael, Robledo-Cadena Diana Xochiquetzal, Pacheco-Velazquez Silvia Cecilia, Rodriguez-Enriquez Sara | |authors= Moreno-Sanchez Rafael, Robledo-Cadena Diana Xochiquetzal, Pacheco-Velazquez Silvia Cecilia, Rodriguez-Enriquez Sara | ||
|year=2022 | |year=2022 | ||
|event=[[Bioblast 2022]] | |event=[[Bioblast 2022]] | ||
|abstract= | |abstract=Glycolytic and respiratory fluxes were analyzed in cancer and non-cancer cells. The steady-state fluxes in energy metabolism were used to estimate the aerobic glycolytic and mitochondrial (oxidative phosphorylation OXPHOS) contributions to the cellular ATP supply. The rate of lactate production, corrected for the fraction generated by glutaminolysis, is proposed as the appropriate way to estimate glycolytic flux. In general, the glycolytic rates estimated for cancer cells are higher than those found in non-cancer cells, as originally observed by Otto Warburg. The rate of ROUTINE ''R'' cellular O<sub>2</sub> consumption corrected for LEAK respiration ''L'' measured after inhibition by oligomycin (a specific, potent and permeable ATP synthase inhibitor) becomes the respiratory ''R-L'' net ROUTINE capacity, which is proposed as the appropriate way to estimate mitochondrial ATP synthesis-linked O<sub>2</sub> flux or net OXPHOS flux in living cells. Detecting non-negligible O<sub>2</sub> consumption rates in cancer cells has revealed that the mitochondrial function is not impaired, as claimed by the Warburg effect. Furthermore, when calculating the relative contributions to cellular ATP supply, under a variety of environmental conditions and for several different types of cancer cells, it was found that OXPHOS was the main ATP provider over glycolysis. Hence, OXPHOS inhibitors can be successfully used to block ATP-dependent processes such as cellular migration in cancer cells. These observations may guide the re-design of novel targeted therapies. | ||
|keywords=Oxidative phosphorylation, oxygen uptake, glycolysis, cell ATP supply, cancer cell migration | |keywords=Oxidative phosphorylation, oxygen uptake, glycolysis, cell ATP supply, cancer cell migration | ||
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== Affiliations == | == Affiliations == | ||
:::: Moreno-SĂĄnchez Rafael<sup>1*</sup>, Robledo-Cadena Diana Xochiquetzal<sup>2</sup>, Pacheco-VelĂĄzquez Silvia Cecilia<sup>2</sup>, RodrĂguez-EnrĂquez Sara<sup>3*</sup> | :::: Moreno-SĂĄnchez Rafael<sup>1*</sup>, Robledo-Cadena Diana Xochiquetzal<sup>2</sup>, Pacheco-VelĂĄzquez Silvia Cecilia<sup>2</sup>, RodrĂguez-EnrĂquez Sara<sup>3*</sup> | ||
::::# | ::::# Facultad de Estudios Superiores Iztacala, UNAM, Tlanepantla, Estado de Mexico, Mexico | ||
::::# Departamento de BioquĂmica, Instituto Nacional de CardiologĂa, Ciudad de MĂ©xico 14080. Mexico | ::::# Departamento de BioquĂmica, Instituto Nacional de CardiologĂa, Ciudad de MĂ©xico 14080. Mexico | ||
::::# Laboratorio de Control MetabĂłlico | ::::# Laboratorio de Control MetabĂłlico, Carrera de Medicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional AutĂłnoma de MĂ©xico; Los Reyes Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Ărboles/Barrio de los HĂ©roes, Tlalnepantla 54090, Mexico | ||
 | == List of abbreviations, terms and definitions - MitoPedia == | ||
== | {{Template:List of abbreviations, terms and definitions - MitoPedia}} | ||
{{Labeling | {{Labeling | ||
| | |event=B2 | ||
|articletype=Abstract | |articletype=Abstract | ||
}} | }} |
Latest revision as of 08:28, 7 September 2022
6.2. «10+5» Moreno-Sanchez Rafael, Robledo-Cadena DX, Pacheco-VelĂĄzquez SC, RodrĂguez-EnrĂquez S (2022) Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect. Bioblast 2022: BEC Inaugural Conference. In: https://doi.org/10.26124/bec:2022-0001 |
Link: Bioblast 2022: BEC Inaugural Conference
Moreno-Sanchez Rafael, Robledo-Cadena Diana Xochiquetzal, Pacheco-Velazquez Silvia Cecilia, Rodriguez-Enriquez Sara (2022)
Event: Bioblast 2022
Glycolytic and respiratory fluxes were analyzed in cancer and non-cancer cells. The steady-state fluxes in energy metabolism were used to estimate the aerobic glycolytic and mitochondrial (oxidative phosphorylation OXPHOS) contributions to the cellular ATP supply. The rate of lactate production, corrected for the fraction generated by glutaminolysis, is proposed as the appropriate way to estimate glycolytic flux. In general, the glycolytic rates estimated for cancer cells are higher than those found in non-cancer cells, as originally observed by Otto Warburg. The rate of ROUTINE R cellular O2 consumption corrected for LEAK respiration L measured after inhibition by oligomycin (a specific, potent and permeable ATP synthase inhibitor) becomes the respiratory R-L net ROUTINE capacity, which is proposed as the appropriate way to estimate mitochondrial ATP synthesis-linked O2 flux or net OXPHOS flux in living cells. Detecting non-negligible O2 consumption rates in cancer cells has revealed that the mitochondrial function is not impaired, as claimed by the Warburg effect. Furthermore, when calculating the relative contributions to cellular ATP supply, under a variety of environmental conditions and for several different types of cancer cells, it was found that OXPHOS was the main ATP provider over glycolysis. Hence, OXPHOS inhibitors can be successfully used to block ATP-dependent processes such as cellular migration in cancer cells. These observations may guide the re-design of novel targeted therapies.
âą Keywords: Oxidative phosphorylation, oxygen uptake, glycolysis, cell ATP supply, cancer cell migration
âą O2k-Network Lab: MX Mexiko City Moreno-Sanchez R
Affiliations
- Moreno-SĂĄnchez Rafael1*, Robledo-Cadena Diana Xochiquetzal2, Pacheco-VelĂĄzquez Silvia Cecilia2, RodrĂguez-EnrĂquez Sara3*
- Facultad de Estudios Superiores Iztacala, UNAM, Tlanepantla, Estado de Mexico, Mexico
- Departamento de BioquĂmica, Instituto Nacional de CardiologĂa, Ciudad de MĂ©xico 14080. Mexico
- Laboratorio de Control MetabĂłlico, Carrera de Medicina, Facultad de Estudios Superiores Iztacala, Universidad Nacional AutĂłnoma de MĂ©xico; Los Reyes Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Ărboles/Barrio de los HĂ©roes, Tlalnepantla 54090, Mexico
- Moreno-SĂĄnchez Rafael1*, Robledo-Cadena Diana Xochiquetzal2, Pacheco-VelĂĄzquez Silvia Cecilia2, RodrĂguez-EnrĂquez Sara3*
List of abbreviations, terms and definitions - MitoPedia
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Event: B2