SUIT-016: Difference between revisions
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::: '''[[SUIT protocol pattern]]:''' diametral/complex 1OctM;2D;3G;4S;5Rot;6Omy;7U | ::: '''[[SUIT protocol pattern]]:''' diametral/complex 1OctM;2D;3G;4S;5Rot;6Omy;7U | ||
__TOC__ | __TOC__ | ||
Β Communicated by [[Doerrier C]] and [[Gnaiger E]] (last update 2019-01- | Β Communicated by [[Doerrier C]], [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-01-30) | ||
== DLP applications == | == DLP applications == | ||
Revision as of 10:41, 30 January 2019
Description
Abbreviation: FNS(Oct,GM)
Reference: A Β»Versions
- SUIT-category: FNS(Oct,GM)
- SUIT protocol pattern: diametral/complex 1OctM;2D;3G;4S;5Rot;6Omy;7U
Communicated by Doerrier C, Huete-Ortega M and Gnaiger E (last update 2019-01-30)
DLP applications
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Gnaiger 2015 Scand J Med Sci Sports | 2015 | Gnaiger E, Boushel R, SΓΈndergaard H, Munch-Andersen T, Damsgaard R, Hagen C, DΓez-SΓ‘nchez C, Ara I, Wright-Paradis C, Schrauwen P, Hesselink M, Calbet JAL, Christiansen M, Helge JW, Saltin B (2015) Mitochondrial coupling and capacity of oxidative phosphorylation in skeletal muscle of Inuit and caucasians in the arctic winter. https://doi.org/10.1111/sms.12612 | Human | Skeletal muscle |
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1OctM | OctML(n) | F(N) | CETF | 1OctM
|
2D | OctMP | F(N) | CETF | 1OctM;2D
|
3G | OctGMP | FN | CETF&I | 1OctM;2D;3G
|
4S | OctGMSP | FNS | CETF&CI&II | 1OctM;2D;3G;4S
|
5Rot | SP | S | CII | 1OctM;2D;3G;4S;5Rot
|
6Omy | SL(Omy) | S | CII | 1OctM;2D;3G;4S;5Rot;6Omy
|
7U | SE | S | CII | 1OctM;2D;3G;4S;5Rot;6Omy;7U
|
8Ama | ROX | 1OctM;2D;3G;4S;5Rot;6Omy;7U;8Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- + This protocol provides information on FAO capacity in the absence of other potentially interfering pathways both in LEAK and OXPHOS coupling control states.
- + FNS OXPHOS capacity comprises the most important pathways in many cell types and thus provides a physiologically relevant estimate of maximum mitochondrial respiratory capacity.
- + FNS ET capacity is a good estimate of overal ET capacity in many cell types.
- + This is a good protocol to analyse coupling control at S-pathway, which is an advantage in relation to SUIT-015 and SUIT-017.
- + Glutamate is easier to prepare compared to pyruvate.
- - SRot(E) may be underestimated if S is not saturating.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
MitoPedia concepts:
MiP concept,
SUIT protocol,
Recommended
MitoPedia methods:
Respirometry