Template talk:Next oroboros ecosystem agenda: Difference between revisions
From Bioblast
No edit summary |
|||
Line 31: | Line 31: | ||
* '''Aug-06 Th''' | * '''Aug-06 Th''' | ||
:{| | |||
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia]] | |||
| OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system, and is the ratio of free to total OXPHOS capacity. It is suggested to use instead of respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling. <br> | |||
<br> | |||
- Β»'''[https://wiki.oroboros.at/index.php/OXPHOS-coupling_efficiency| OXPHOS-coupling efficiency]'''Β« | |||
|} | |||
== O2k-Brief alert == | == O2k-Brief alert == | ||
Revision as of 16:27, 27 July 2020
- Antunes Diana (talk) 08:58, 5 May 2020 (CEST) Format for new Agenda/Alerts entries:
{| | logos | title in bold <br> - link |}
e.g.,
Did you know that the proper use of TIP2k-Filter Papers may avoid an unstable O2 slope neg. signal during Instrumental background correction?
- Β»Instrumental backgroundΒ« and Β»TIP2k Filter PapersΒ«
- See the scheme:
Bioblast alert 2020
Prep for circular done here: Talk:Bioblast alert 2020
MitoPedia alert
Next
- Aug-06 Th
OXPHOS-coupling efficiency (1-L/P) describes the coupling of the mitochondrial electron transfer system, and is the ratio of free to total OXPHOS capacity. It is suggested to use instead of respiratory acceptor control ratio (RCR) to evaluate mitochondrial coupling.
O2k-Brief alert
Next
O2k-Publications alert
Next
- Aug-04 Tu
Deletion of Prkn impairs mitochondrial respiratory capacity: a non-alcoholic fatty liver disease pathogenesis
- from the O2k-Network Β»US PA Pittsburgh Jurczak MJΒ«
- Edmunds LR, Xie B, Mills AM, Huckestein BR, Undamatla R, Murali A, Pangburn MM, Martin J, Sipula I, Kaufman BA, Scott I, Jurczak MJ (2020) Liver-specific Prkn knockout mice are more susceptible to diet-induced hepatic steatosis and insulin resistance. Mol Metab [Epub ahead of print]. Β»Bioblast linkΒ«
O2k-Open Support alert
Next
- While in Home Office: Gnaigere (talk) 14:03, 19 March 2020 (CET) https://wiki.oroboros.at/index.php/Talk:O2k-Open_Support_alert - Many labs will move to home office mode. In line with our special DL7.4 home office offer (see Agenda today), therefore, I suggest to post in our Agenda analysis-linked O2k-Open Support cases during this time, and collect real-time operation cases for future releases.
- Aug-10 Mo
Removed for correction
Do you perform the leaky chamber test after chamber assembly?
An unnoticed leak from the chamber may affect your data, therefore we recommend performing the Leaky chamber test - especially after chamber reassembly.
- Β»Leaky chamber testΒ« and Β» https://intern.oroboros.at/index.php/Overnight_test_after_O2k-chamber_assemblyΒ«
O2k-Open Support alert circulars
O2k-Open Support alert circular 2020(07): in prep
Do you know how to select the proper Fluorescence-Sensor and filter set?
Using different Fluorescence-Sensors and Filters, the O2k-Fluo Smart-Module allows for measurement of Hydrogen peroxide, ATP, Mitochondrial membrane potential, Calcium, and numerous other applications open for O2k-user innovation.
- Β»Selecting the Smart Fluo-SensorΒ« and Β»Setup of the O2k-Fluo LED2-ModuleΒ«
Do you know the different ways to adjust oxygen concentration in the Oroboros O2k?
Did you know that some chemicals are incompatible with the Amplex UltraRed assay?
- Β»Incompatible substratesΒ«
In preparation
For discussion
Here we discuss suggestions for the Oroboros Ecosystem Agenda 2020 (if the specific alert section is yet to be attributed)
Controlled stability of the MitoKit-CII based on HPLC and LCMS analysis: No degradation of MitoKit-CII compounds MitoKit-CII/Succinate-nv (NV118) and MitoKit-CII/Malonate-nv (NV161) for following storage conditions: - Proven -80 Β°C solid stability for more than 3 years (3 years 7 months)
- Proven -20 Β°C solid stability for 1 year
- Proven DMSO stability at room temperature for 1 week
- Proven DMSO stability at -20 Β°C for 6 months (ongoing)
2021
- 2021-01-06 https://www.jstor.org/stable/1706346?seq=1