MiR05: Difference between revisions
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|description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.ย [[MiR06]] = MiR05 + catalase. | |description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.ย [[MiR06]] = MiR05 + catalase. | ||
[[MiR05Cr]] = [[MiR05]] + creatine. | [[MiR05Cr]] = [[MiR05]] + creatine. | ||
|info=[ | |info=[https://www.oroboros.at/index.php/product/mir05-kit/ MiR05-Kit], [[Gnaiger 2000 Life in the Cold]] | ||
}} | }} | ||
== Comparison of respiration media == | == Comparison of respiration media == | ||
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== MiR05-Kit in HRR == | == MiR05-Kit in HRR == | ||
::::* For preparation of MiR05, see [[MiR05- | ::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-kit]]. | ||
::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''. | ::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''. | ||
Revision as of 12:07, 11 August 2020
Description
[[Description::Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. Respiration of living cells may be assessed in MiR05 by adding pyruvate (P) as an external source. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.]]
Abbreviation: MiR05
Reference: [[Info::MiR05-Kit, Gnaiger 2000 Life in the Cold]]
Comparison of respiration media
- Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - ยปBioblast linkยซ - The original article describing MiR05 in comparison with the composition of mitochondrial and organ preservation solutions.
- Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - ยปBioblast linkยซ
- Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - ยปBioblast linkยซ - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
- Komlรณdi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - ยปBioblast linkยซ
- Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mรฉszรกros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - ยปBioblast linkยซ
MiR05-Kit in HRR
- For preparation of MiR05, see MiPNet22.10 MiR05-kit.
- The oxygen solubility factor of MiR05 is 0.92.
MitoPedia topics:
Media for respirometry