From Bioblast
Description
Abbreviation: NS(PGM)
Reference: A: SUIT 8 is the SUIT protocol used in the MitoEAGLE project (WG2) for testing the skill preparing permeabilized fibers Lemieux 2017 Sci Rep
SUIT number: 8
MitoPedia concepts:
SUIT protocol,
SUIT A
- SUIT-category: NS(PGM)
- SUIT protocol pattern: diametral.
1PM;2D;2c;3G;4S;5U;6Rot-
Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
---|---|---|---|---|
1PM | PM(L) | N | CI | |
2D | PM(P) | N | CI | |
2D(2c) | PM(c) | N | CI | |
3G | PGM(P) | N | CI | |
4S | PGMS(P) | NS | CI&II | |
5U | PGMS(E) | NS | CI&II | |
6Rot | S(E) | S | CII | |
7Ama | ROX |
1PM;2D;3G;3c;4S;5U;6Rot-
Step | Respiratory state | Pathway control | Entry through electron transfer Complex | Comments |
---|---|---|---|---|
1PM | PM(L) | N | CI | |
2D | PM(P) | N | CI | |
3G | PGM(P) | N | CI | |
G(c) | PGM(c) | N | CI | Limitation: Cytochrome c should be added after step 2D, such that all OXPHOS states can be compared in the presence of cytochrome c. See 1PM;2D;2c;3G;4S;5U;6Rot-. |
4S | PGMS(P) | NS | CI&II | |
5U | PGMS(E) | NS | CI&II | |
6Rot | S(E) | S | CII | |
7Ama | ROX |
SUIT O2 DLP for SUIT 8 (DatLab 7.1)
- DL-Protocols (DLP) for substrate-uncoupler-inhibitor titrations (see MitoPedia: SUIT) guide through a sequence of coupling and pathway control states. A specific SUIT protocol can be assigned to O2k-Chamber A or B or both. DL-Protocols for different SUIT protocols can be found in the library of protocols MitoPedia: SUIT. Instrumental DL-protocols are displayed in the DL-Protocol library.
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References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Lemieux 2017 Sci Rep | 2017 | Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electron flow through the Q-junction in permeabilized fibers. Sci Rep 7:2840. doi:10.1038/s41598-017-02789-8 | Mouse | Heart |