E-L net ET capacity: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=''โE'' | |abbr=''โE'' | ||
|description=[[Image:E-L.jpg|50 px|Free ETS capacity]] The '''free ETS capacity''', ''โE'', is the [[ETS capacity]] corrected for [[LEAK respiration]], ''โE'' = ''E-L''. ''โE'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ETS state, therefore, is partitioned into the free ETS capacity, ''โE'', and | |description=[[Image:E-L.jpg|50 px|Free ETS capacity]] The '''free ETS capacity''', ''โE'', is the [[ETS capacity]] corrected for [[LEAK respiration]], ''โE'' = ''E-L''. ''โE'' is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ETS state, therefore, is partitioned into the free ETS capacity, ''โE'', and LEAK respiration, ''L<sub>P</sub>'', compensating for proton leaks, slip and cation cycling: ''E'' = ''โE''+''L<sub>P</sub>'' (see [[free OXPHOS capacity]]). | ||
|info=[[Gnaiger 2014 MitoPathways]] | |info=[[Gnaiger 2014 MitoPathways]] | ||
}} | }} |
Revision as of 05:17, 12 March 2015
Description
The free ETS capacity, โE, is the ETS capacity corrected for LEAK respiration, โE = E-L. โE is the respiratory capacity potentially available for ion transport and phosphorylation of ADP to ATP. Oxygen consumption in the ETS state, therefore, is partitioned into the free ETS capacity, โE, and LEAK respiration, LP, compensating for proton leaks, slip and cation cycling: E = โE+LP (see free OXPHOS capacity).
Abbreviation: โE
Reference: Gnaiger 2014 MitoPathways
MitoPedia methods:
Respirometry
MitoPedia topics: "Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Respiratory state"Respiratory state" is not in the list (Enzyme, Medium, Inhibitor, Substrate and metabolite, Uncoupler, Sample preparation, Permeabilization agent, EAGLE, MitoGlobal Organizations, MitoGlobal Centres, ...) of allowed values for the "MitoPedia topic" property.
Coupling control states for โE
- Reference state, ZX: ETS capacity, E = Eยด-ROX
- Background state, YX: LEAK respiration, L = Lยด-ROX
- Metabolic control variable, X=ZX-YX: Scope of uncoupler stimulation, free ETS capacity, โE = E-L
Flux control factor
- ยป Flux control factor, FCF
- Coupling control factor, 1-YX/ZX: E-L coupling control factor, ETS coupling efficiency: jโE = โE/E =(E-L)/E = 1-L/E
Compare
- mt-Preparations Free OXPHOS capacity, โP = P-L
- Intact cells Free ROUTINE activity, โR = R-L