Horseradish peroxidase: Difference between revisions
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Revision as of 15:07, 24 May 2012
- high-resolution terminology - matching measurements at high-resolution
Horseradish peroxidase
Description
Horseradish peroxidase readily combines with hydrogen peroxide (H2O2) and the resultant [HRP-H2O2] complex can oxidize a wide variety of hydrogen donors.
Abbreviation: HRP
MitoPedia methods:
Fluorometry
Application in HRR
HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C
Reaction
Donor + H2O2 → Oxidized Donor + 2 H2O
Comment
The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by Laszlo Tretter.
