ISE-Ca2+ Membranes: Difference between revisions
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{{ | {{Technical support}} | ||
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|description=[[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes. | |description=[[Image:Ca2+ membranes.jpg|right|180px]]'''ISE-Ca2+ Membranes''': PVC, 4 mm diameter, box of 5 membranes. | ||
To be used with the [[O2k-TPP+ ISE-Module]]. ย | To be used with the [[O2k-TPP+ ISE-Module]]. ย | ||
|info= [ | |info= [https://www.oroboros.at/index.php/product/ise-ca2-membranes/ '''Product details and purchase information'''] | ||
}} | }} | ||
== Technical support == | == Technical support == | ||
:::: The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al<ref>Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca<sup>2+</sup>-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.</ref>. The limit of detection is less than 10 nM in Ca<sup>2+</sup> buffered calibration solutions. The main difficulty in establishing reliable Ca<sup>2+</sup> measurements is not the Ca<sup>2+</sup> sensitive membrane but the methodology of its application in a medium simulating intracellular conditions | :::: The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al<ref>Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca<sup>2+</sup>-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.</ref>. The limit of detection is less than 10 nM in Ca<sup>2+</sup> buffered calibration solutions. The main difficulty in establishing reliable Ca<sup>2+</sup> measurements is not the Ca<sup>2+</sup> sensitive membrane but the methodology of its application in a medium simulating intracellular conditions. ย | ||
== Additional resources == | |||
:::ยป [[MiPNet15.03 O2k-MultiSensor-ISE|ISE Manual]] | |||
:::ยป [[O2k-TPP+ ISE-Module]] | |||
== References == | |||
<references/> | |||
{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry | ||
|mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry | |mitopedia O2k and high-resolution respirometry=O2k hardware, O2k-Open Support, O2k-Respirometry, O2k-FluoRespirometry | ||
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Latest revision as of 11:50, 11 November 2020
ISE-Ca2+ Membranes |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
ISE-Ca2+ Membranes: PVC, 4 mm diameter, box of 5 membranes.
To be used with the O2k-TPP+ ISE-Module.
Reference: Product details and purchase information
Technical support
- The composition of the ISE-Ca2+ Membranes is similar to the one described by Simon et al[1]. The limit of detection is less than 10 nM in Ca2+ buffered calibration solutions. The main difficulty in establishing reliable Ca2+ measurements is not the Ca2+ sensitive membrane but the methodology of its application in a medium simulating intracellular conditions.
Additional resources
References
- โ Schefer U, Ammann D, Pretsch E, Oesch U, Simon W (1986) Neutral carrier based Ca2+-selective electrode with detection limit in the sub-nanomolar range. Analyt Chem 58:2282-5.
MitoPedia O2k and high-resolution respirometry:
O2k hardware,
O2k-Open Support,
O2k-Respirometry,
O2k-FluoRespirometry