MiPNet03.02 Chemicals-Media: Difference between revisions
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{{Publication | {{Publication | ||
|title=Selected media and chemicals for respirometry with mitochondrial preparations. | |title=Selected media and chemicals for respirometry with mitochondrial preparations. [[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/3/3c/MiPNet03.02_Chemicals-Media.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet03.02_Chemicals-Media.pdf Versions | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/3/3c/MiPNet03.02_Chemicals-Media.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet03.02_Chemicals-Media.pdf Versions] | ||
|authors=Oroboros | |authors=Oroboros | ||
|year=2016-08-30 | |year=2016-08-30 | ||
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== Cited by == | == Cited by == | ||
{{Template:Cited by Cardoso 2021 MitoFit MgG}} | {{Template:Cited by Cardoso 2021 MitoFit MgG}} | ||
{{Template:Cited by Komlodi 2021 MitoFit Tissue normoxia}} | |||
{{Labeling | {{Labeling | ||
|area=Instruments;methods | |area=Instruments;methods | ||
|instruments=O2k-Protocol | |instruments=O2k-Protocol | ||
|additional=O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG | |additional=O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH | ||
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Latest revision as of 17:38, 17 October 2023
MiPNet03.02 Chemicals-Media
Selected media and chemicals for respirometry with mitochondrial preparations.  |
» 
»Versions
Oroboros (2016-08-30) Mitochondr Physiol Network
Abstract: Fontana-Ayoub M, Fasching M, Gnaiger E (2016) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(18):1-10. Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initial decisions on the composition of media and chemicals are decisive for long-term studies and crucial for comparability of results. As a guideline, we summarize an update of our experience with media and chemicals for high-resolution respirometry with isolated mitochondria, permeabilized cells, muscle fibres and tissue homogenates. Whereas optimization is necessary for specific experimental protocols, standardization will improve the comparability of results obtained in different laboratories. Efforts towards standardization are important for the advancement of mitochondrial physiology.
- » Product: Oroboros O2k, O2k-Catalogue
• O2k-Network Lab: AT Innsbruck Oroboros
Supplementary information
- » MiPNet09.12 O2k-Titrations; MitoPedia
- » Cytochrome c control factor
- » For calculations, see Excel file: O2k-Titrations.xls
- » For pH adjustment of BIOPS solution, note the temperature dependence:
- at 0 °C pH = 7.1
- at 23 °C pH = 6.75
Cited by
- Cardoso et al (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. doi:10.26124/bec:2021-0001
- Komlodi et al (2022) Hydrogen peroxide production, mitochondrial membrane potential and the coenzyme Q redox state measured at tissue normoxia and experimental hyperoxia in heart mitochondria. MitoFit Preprints 2021 (in prep)
Labels: MiParea: Instruments;methods
HRR: O2k-Protocol
O2k-chemicals and media, Uncoupling, ADP, ATP, MitoFit 2021 MgG, MitoFit 2021 Tissue normoxia, MitoFit 2022 NADH
