MiPNet06.03 POS-calibration-SOP: Difference between revisions
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{{Publication | {{Publication | ||
|title=Gnaiger E (2014) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(12): 1- | |title=Fasching M, Gnaiger E (2014) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(12): 1-8. | ||
|info=[[Media:MiPNet06.03 POS-Calibration-SOP.pdf|'''Open Access-MiPNet06.03.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]ย [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | |info=[[Media:MiPNet06.03 POS-Calibration-SOP.pdf|'''Open Access-MiPNet06.03.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet06.03_POS-Calibration-SOP.pdf Versions]ย [[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]] | ||
|authors=OROBOROS | |authors=OROBOROS |
Revision as of 13:57, 21 February 2014
Fasching M, Gnaiger E (2014) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(12): 1-8. |
ยป Open Access-MiPNet06.03.pdf; Versions
OROBOROS (2014) Mitochondr Physiol Network
Abstract: High-resolution respirometry critically depends on accurate calibration of the polarographic oxygen sensors which respond to partial oxygen pressure. Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The oxygen solubility of mitochondrial respiration medium MiR06 relative to pure water (oxygen solubility factor, FM) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; MiPNet14.13) at 37 ยฐC and 30 ยฐC. At air saturation at standard barometric pressure (100 kPa) and 37 ยฐC, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 ยตM in MiR06. A clean and ready-to-use Oxygraph chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
- >> O2k-Protocols:Overall contents
- >> Product: OROBOROS Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP
Further details
- >> MiPNet12.08 O2k-Calibration
- >> MiPNet14.06 InstrumentalBackground
- >> Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York: 321-333.