Difference between revisions of "SUIT-006"
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{{MitoPedia | {{MitoPedia | ||
|abbr= | |abbr=N or S | ||
|description=[[File: | |description=[[File:|200px]] | ||
|info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-006.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-006.pdf Versions] | |info='''A''' [[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/6/64/SUIT-006.pdf|Bioblast pdf]] »[http://wiki.oroboros.at/index.php/File:SUIT-006.pdf Versions] | ||
}} | }} | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' N or S | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' linear [[coupling control protocol]] 1X;2D;3U;- | ||
__TOC__ | __TOC__ | ||
== DLP applications == | == DLP applications == | ||
::::* [[SUIT-006 O2 | ::::* [[SUIT-006 O2]] | ||
== References == | == References == | ||
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== Strengths and limitations == | == Strengths and limitations == | ||
:::* This protocol is indicated when the aim is to analyse coupling control in mt preparations. | |||
:::* Different combinations of substrates and inhibitors can be used depending on the specific aims, e.g.: PM, GM, MnaPM or other combinations for N-pathway control state; S or RotS for S-pathway control state. | |||
:::+ The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state. | |||
:::+ Short duration of the experiment. | |||
:::- Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler. | |||
:::- CIV activity is not measured, to save experimental time. | |||
Revision as of 11:35, 17 January 2019
Description
[[File:|200px]]
Abbreviation: N or S
Reference: A »Versions
- SUIT-category: N or S
- SUIT protocol pattern: linear coupling control protocol 1X;2D;3U;-
DLP applications
References
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1X | XL(n) | 1X
| ||
2D | XP | 1X;2D
| ||
2c | XcP | 1X;2D;2c
| ||
3Omy | XLOmy | 1X;2D;2c;3Omy
| ||
4U | XE | 1X;2D;2c;3Omy;4U
| ||
5Ama | ROX | 1X;2D;2c;3Omy;4U;5Ama
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- This protocol is indicated when the aim is to analyse coupling control in mt preparations.
- Different combinations of substrates and inhibitors can be used depending on the specific aims, e.g.: PM, GM, MnaPM or other combinations for N-pathway control state; S or RotS for S-pathway control state.
- + The combinations of substrates for one ET-pathway state with inhibitors for other pathways provide the best coupling control protocols specififc for one ET-pathway state, e.g.: MnaPM for N-pathway control state, RotS for S-pathway control state.
- + Short duration of the experiment.
- - Careful washing is required after the experiment to avoid carry-over of inhibitors and uncoupler.
- - CIV activity is not measured, to save experimental time.
Compare SUIT protocols
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry