Difference between revisions of "SUIT-017 O2 pfi D049"
From Bioblast
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::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' FNS(Oct,GM) | ||
::: '''[[SUIT protocol pattern]]:''' diametral 1OctM;2D;2c;3G;4S;5U;6Rot- | ::: '''[[SUIT protocol pattern]]:''' diametral 1OctM;2D;2c;3G;4S;5U;6Rot- | ||
SUIT-017 O2 pfi D049 gives information on [[Fatty_acid_oxidation_pathway_control_state|F-pathway]] in [[LEAK-respiration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]] avoiding FAO overestimation in the presence of [[Anaplerosis|anaplerotic]] pathways. In addition, the pathway control of [[FN]] and [[FNS]] in [[Oxidative phosphorylation|OXPHOS state]] and of [[FNS]] and [[S]] in [[ET-capacity| ET state]] is evaluated. | |||
__TOC__ | __TOC__ | ||
Communicated by [[Cardoso LH]] and [[Gnaiger E]] (last update 2019-02-26) | Communicated by [[Cardoso LH]] and [[Gnaiger E]] (last update 2019-02-26) | ||
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::::+ This protocol can be extended with the Complex IV module, which can prolong the experimental time with ~30 min. | ::::+ This protocol can be extended with the Complex IV module, which can prolong the experimental time with ~30 min. | ||
== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT-002]] | ::::* [[SUIT-002]]: A more comprehensive protocol incuding additional substrate states. | ||
::::* [[SUIT-005]] | ::::* [[SUIT-005]]: A comparable protocol evaluating P instead of G. | ||
== References == | == References == |
Revision as of 13:27, 3 April 2019
Description
Abbreviation: FNS(Oct,GM)
Reference: A: SUIT-017
SUIT number: D049_1OctM;2D;2c;3G;4S;5U;6Rot;7Ama
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: FNS(Oct,GM)
- SUIT protocol pattern: diametral 1OctM;2D;2c;3G;4S;5U;6Rot-
SUIT-017 O2 pfi D049 gives information on F-pathway in LEAK state and OXPHOS state avoiding FAO overestimation in the presence of anaplerotic pathways. In addition, the pathway control of FN and FNS in OXPHOS state and of FNS and S in ET state is evaluated.
Communicated by Cardoso LH and Gnaiger E (last update 2019-02-26)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1OctM | OctML(n) | F(N) | FAO | 1OctM
|
2D | OctMP | F(N) | FAO | 1OctM;2D
|
3G | OctGMP | FN | F&CI | 1OctM;2D;3G
|
4S | OctGMSP | FNS | F&CI&II | 1OctM;2D;3G;4S
|
5U | OctGMSE | FNS | F&CI&II | 1OctM;2D;3G;4S;5U
|
6Rot | SE | S | CII | 1OctM;2D;3G;4S;5U;6Rot
|
7Ama | ROX | 1OctM;2D;3G;4S;5U;6Rot;7Ama
|
Step | Respiratory state | Pathway control | ET-Complex | Comment |
---|---|---|---|---|
## AsTm | AsTmE | CIV | CIV | |
## Azd | CHB |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- Comparison of GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI (Lemieux et al. 2017; Votion et al. 2012).
- + Glutamate is easier to prepare compared to pyruvate.
- + Reasonable duration of the experiment.
- + This protocol can be extended with the Complex IV module, which can prolong the experimental time with ~30 min.
Compare SUIT protocols
References
Year | Reference | Organism | Tissue;cell | |
---|---|---|---|---|
Pesta 2012 Methods Mol Biol | 2012 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Human | Skeletal muscle Other cell lines HEK Fibroblast HUVEC |
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry