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...of mitochondrial subpopulations from skeletal muscle: Optimizing recovery and preserving integrity. Acta Physiol (Oxf) 225:13182. ...ehensive approach is developed to identify the best conditions to separate mitochondrial fractions.

|title=Selected media and chemicals for respirometry with mitochondrial preparations. [[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-P ...ing M, Gnaiger E (2016) Selected media and chemicals for respirometry with mitochondrial preparations. Mitochondr Physiol Network 03.02(18):1-10.'''

...s outputs with hypoxic exposure, and this may protect cardiac bioenergetic function in hypoxic tropical waters. |preparations=Permeabilized tissue

...cobs R, Lundby C (2014) Hypoxic training: effect on mitochondrial function and aerobic performance in hypoxia. Med Sci Sports Exerc 46:1936-45. ...ude, and ii) the benefits are primarily mediated by improved mitochondrial function of skeletal muscle.

...(2013) The effects of hypoxic training on aerobic performance in normoxia and moderate hypoxia: a randomized, double blind, placebo controlled study. Mit ...raining conferred no advantage on VO2max, but improved cycling efficiency, and tended to enhance time trial performance (52 vs 32%, P=0.09). Our data sugg

...d exposure to hypoxia and inactivity on oxidative function ''in vivo'' and mitochondrial respiration ''ex vivo'' in humans. J Appl Physiol (1985) 121:154-63. ...not aggravate, and partially reversed, the impairment of muscle oxidative function ''in vivo'' induced by bed rest. The effects of longer exposures will have

...ntary humans. A respiratory study on permeabilized muscle fibers. Abstract Mitochondrial Medicine Chicago. |event=Mitochondrial Medicine Chicago

...impact of postnatal hyperoxia exposure on RV hemodynamic and mitochondrial function across the lifespan. ...loops and by high-resolution respirometry at postnatal day 21 (P21), P90, and P365.

...me TA, Eaton S, Szibor M, Viscomi C, James AM, Murphy MP (2018) Control of mitochondrial superoxide production by reverse electron transport at complex I. J Biol Ch ...CoQ redox state, indicating that complex I RET represents a major mode of mitochondrial redox signaling.

...iPsocietyLOGO.JPG|left|100px|Mitochondrial Physiology Society|MiPsociety]] Mitochondrial physiology in cardiac muscle of deer mice native to high altitude. ...irculatory O₂ delivery by the heart is essential for supporting tissue O₂ demands, but it is unclear whether evolved or plastic changes

...yons SA, Henry DA, Scott GR (2018) Effects of chronic hypoxia on diaphragm function in deer mice native to high altitude. Acta Physiol (Oxf) 223:e13030. ...=We examined the effects of chronic hypoxia on diaphragm function in high- and low-altitude populations of Peromyscus mice.

...omparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. ...Taken together, MiR05 and Buffer Z yield comparable results on respiration and H2O2 production. Despite the lower sensitivity, MiR05 was selected as the m

|title=Pamenter ME, Gomez CR, Richards JG, Milsom WK (2016) Mitochondrial responses to prolonged anoxia in brain of red-eared slider turtles. Biol Le ..., (ii) markedly reduced aerobic capacity, and (iii) mild uncoupling of the mitochondrial proton gradient. These data suggest that turtle brain mitochondria respond

...Lau GY, Richards JG (2013) Beating oxygen: chronic anoxia exposure reduces mitochondrial F1FO-ATPase activity in turtle (''Trachemys scripta'') heart. J Exp Biol 21 ...eduction in Complex V activity is a fundamental component of mitochondrial and cellular anoxia survival.

...tochondrial physiology and capacity of oxidative phosphorylation in active and sedentary life styles. Abstract Targeting Mitochondria Berlin. ...itions (37 °C; Complex I+II substrate combination) with minimal amounts of tissue biopsy (1 to 3 mg wet weight per assay) [2].

|title=Mitochondrial inefficiencies and anoxic ATP hydrolysis capacities in diabetic rat heart. ...concentrations. Whereas diabetic cardiac mitochondria produce less ATP in normoxia, they consume as much ATP in anoxic infarct-like states (Fig. 1).

...gy and function. The aim of this study was to examine cardiac mitochondria function in an avian model, the chicken, in response to embryonic development under ...ction were measured in permeabilized cardiac fibers from externally pipped and 1-day post hatchlings.

|title=Gnaiger E, Méndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Nat ...importance of low intracellular oxygen levels in avoiding oxidative stress and protecting bioenergetic efficiency.

...Markworth JF, Edge JA, Hickey AJ (2015) Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high i ...tion remains poorly studied in humans. Mitochondrial metabolism as well as mitochondrial-associated protein expression were tested in untrained participants perform

...d inspired oxygen influence oxygen extraction at maximal exercise: Role of mitochondrial oxygen affinity. Acta Physiol (Oxf) 225:e13110. ...ke during exercise with large and small muscle mass during normoxia (NORM) and hyperoxia (HYPER).

...ssment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12- ...rophore applied for probing mtMP, in any respiratory state, type of tissue and pathophysiological condition.

|title=Pamenter ME, Gomez CR, Richards JG, Milsom WK (2021) Mitochondrial responses to prolonged anoxia in brain of red-eared slider turtles. Biol Le |abstract=Mitochondria are central to aerobic energy production and play a key role in

|title=[[File:MarkovaM.jpg|left|90px|Michaela Markova]] Mitochondrial respiration in the rat left heart ventricle after inhibition of pyruvate de ...H kinase inhibitor – DCA (dichloroacetic acid) ''in vivo'' during normoxia and anoxia.

...al respiration and hydrogen peroxide production under hyperoxic, normoxic, and hypoxic conditions. '''Bioblast 2022: BEC Inaugural Conference.''' In: http ...th cultured cells and mitochondrial preparations to simulate extracellular and intracellular conditions in the tissues of origin.

...land GB, Scott GR (2017) Evolved changes in the intracellular distribution and physiology of muscle mitochondria in high-altitude native deer mice. J Phys ...ly provide a better indication of adaptive traits that improve performance and hypoxia resistance at high altitudes.

...al sample is relevant for specific applications (''e.g.'', hypoxia, tissue normoxia, high oxygen levels for working with [[permeabilized muscle fibers]]) or si ...TIP2k |TIP2k microsyringes]]. This method is called the "oxystat" approach and supplies an appropriate template for using the TIP2k to control oxygen conc

|title=Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Am ...t is specific to H₂O₂, can be calibrated accurately, and allows continuous real-time measurement. Without correction for the backgro

...increased NADH pool oxidation and lower ROS in COX4i2-expressing cells in normoxia. Most remarkable changes were uncovered in COX oxygen kinetics. The p50 (pa

...increased NADH pool oxidation and lower ROS in COX4i2-expressing cells in normoxia. Most remarkable changes were uncovered in COX oxygen kinetics. The p50 (pa

|title=[[Image:MiPsocietyLOGO.JPG|left|90px|Mitochondrial Physiology Society|MiPsociety]] Neuroprotective compound R-phenibut protect ...The aim of the present study was to evaluate the effects of R-phenibut on mitochondrial functionality in ''in vitro'' model of anoxia-reoxygenation.

...iger E (2003) Oxygen conformance of cellular respiration. A perspective of mitochondrial physiology. https://doi.org/10.1007/978-1-4419-8997-0_4 ...considering the kinetic effects of CIV excess capacity on flux through the mitochondrial electron transfer system.

...ocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007 ...de the unique experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry.

...Abstract FEPS, Santiago de Compostela, Spain. Symposium: Advances in Aging and Mitochondria Research. September 08-12. |info=[http://www.feps2012.org/ Joint FEPS and Spanish Physiol Soc Scientific Congress 2012]

...r-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. https://doi.org/10.1007/BF02111656 ...mes, insufficient attention is paid to the notion of complementary kinetic and thermodynamic flux control mechanisms.<br>

==''MitoCom lectures'': Introduction to mitochondrial respiratory physiology - Innsbruck, November 2011== ...Lectures will be complemented by group discussions of relevant literature and experimental progress.

...However, the functional impact of the isoform switch for mammalian tissues and cells is not fully understood yet. ...hese cells were subsequently utilized as a platform for knock-in of COX4-1 and COX4-2 variants using stable overexpression of either variant from pcDNA 3.

...e separation of '''qualitative versus quantitative change of mitochondrial function''' is addressed in a new O2k-Publication. Does training induce mild uncoupl ...hondrial respiration following strength and endurance training in normoxia and hypoxia in sedentary humans. Am J Physiol Regul Integr Comp Physiol 301: R1

...apacity, using high-resolution respirometry in permeabilized muscle fibres and enzyme activity assays. ...I-linked OXPHOS capacity (''P''<0.05). LEAK to OXPHOS ratio, a measure of mitochondrial uncoupling, was unaffected by hypoxia.

...tion with '''[[O2k-Network Labs]]''' as a service for the O2k-Network Lab, and feedback support for Oroboros Instruments to disseminate the newest develop # Setting up the O2k and instrumental service.

...t' stands for disposable multiwell systems - expensive, with limited scope and extremely high running costs. In respirometry, high throughput is not equiv {{MitoPedia O2k and high-resolution respirometry

...]] of cytochrome c oxidase, '''[[basal metabolic rate]]''', [[P/O ratio]]s and ergometric [[efficiency]]: ...arsen FJ, Schiffer TA, Sahlin K, Ekblom B, Weitzberg E, Lundberg JO (2011) Mitochondrial oxygen affinity predicts basal metabolic rate in humans. FASEB J 25: 2843-5

...aiger.jpg|left|66px|Erich Gnaiger]] OXPHOS capacity in human muscle tissue and body mass excess – the MitoEAGLE mission towards an integrative database ...of tissue-specific to systemic mitochondrial fitness from muscle to brain; and (''6'') documents the added value of Open Access data repositories.

..., Wright-Paradis C, Sondergaard H, Ara I, Helge JW, Saltin B (2011) Muscle mitochondrial capacity exceeds maximal oxygen delivery in humans. Mitochondrion 11:303-7. ...ntained peak leg and pulmonary VO2 despite substantial reduction in muscle mitochondrial capacity. Scand J Med Sci Sports 25 (Suppl 4):135–43. - [[Boushel 2015 Sc

...rg|MiPsociety]]Laner V, Bidaurratzaga E, Gnaiger E, eds (2013) Comparative Mitochondrial Physiology. MiP2013. Mitochondr Physiol Network 18.08:96 pp. ISBN 978-3-950 ...08 MiP2013|'''<big>9^th MiP''conference'' - MiP2013: Comparative Mitochondrial Physiology</big>''']]

::::* 16:00-16:30 [[Stefan_E|Eduard Stefan]]: “Decoding and Perturbation of ONCO-Kinase functions” ...]]: “[[MitoKIN]] - Systematic barcoding of kinase-controlled mitochondrial function”

...2024]] - contact for EBEC 2024: [mailto:Mito-and[email protected] Mito-and[email protected]] Chair [[Mitochondrial Physiology Society]]

'''In: Gnaiger E, ed (2012) Mitochondrial Pathways and Respiratory Control. 3rd ed, OROBOROS MiPNet Publications, Innsbruck''' *Cell Respiration and Phosphorylation Control: [[MiPNet08.09]]

Gnaiger E (2012) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 3rd ed. Mitochondr = Bioblast online references and notes =

Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4^th ed. = Bioblast online references and notes =

...chondrial electron transfer system transfers electrons from dehydrogenases and respiratory complexes downstream to Complex III. <br> - communicated by »[[Komlodi Timea]]« and »[[Schmitt Sabine]]« <br>

|title=Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. ...in and muscle function, and resistance against preventable, immunological, and age-related degenerative diseases?

: <big><big><big>'''Mitochondrial physiology'''</big></big></big> |title=Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1. https://doi.org/10.26124/bec:2020-0001.