Substrate-uncoupler-inhibitor titration: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|abbr=SUIT | |abbr=SUIT | ||
|description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') protocols are used with [[mt-preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]]. | |description=Mitochondrial '''Substrate-uncoupler-inhibitor titration''' ('''SUIT''') [[MitoPedia: SUIT |protocols]] are used with [[mt-preparations]] to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental [[assay]]. | ||
|info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]] | |info=[[Gnaiger 2014 MitoPathways]], [[MiPNet09.12]], [[MitoPedia: SUIT]] | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
{{MitoPedia concepts | {{MitoPedia concepts | ||
|mitopedia concept= | |mitopedia concept=MiP concept, SUIT concept | ||
|type=Respiration | |type=Respiration | ||
}} | }} | ||
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|type=Respiration | |type=Respiration | ||
}} | }} | ||
{{MitoPedia O2k and high-resolution respirometry | {{MitoPedia O2k and high-resolution respirometry|type=Respiration | ||
|type=Respiration | |||
}} | }} | ||
{{MitoPedia topics | {{MitoPedia topics | ||
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|type=Respiration | |type=Respiration | ||
}} | }} | ||
Contributed by [[Gnaiger E]] 2010-09-18, edited 2016-01-24. | |||
[[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | [[File:Pesta_2012_Methods_Mol_Biol_Fig7A.jpg|right|500px|link=Pesta_2012_Methods_Mol_Biol|Pesta and Gnaiger 2012]] | ||
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:::: Attempts are underway to develop a database of SUIT protocols: the [[SUIT protocol library]]. | :::: Attempts are underway to develop a database of SUIT protocols: the [[SUIT protocol library]]. | ||
==References== | |||
== References == | |||
::::* [[Boushel 2007 Diabetologia]] | ::::* [[Boushel 2007 Diabetologia]] | ||
::::* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]] | ::::* [[Gnaiger 2007 MitoPathways]] » ''new edition:'' [[Gnaiger 2014 MitoPathways]] | ||
Revision as of 08:50, 15 May 2016
- high-resolution terminology - matching measurements at high-resolution
Substrate-uncoupler-inhibitor titration
Description
Mitochondrial Substrate-uncoupler-inhibitor titration (SUIT) protocols are used with mt-preparations to study respiratory control in a sequence of coupling and substrates states induced by multiple titrations within a single experimental assay.
Abbreviation: SUIT
Reference: Gnaiger 2014 MitoPathways, MiPNet09.12, MitoPedia: SUIT
MitoPedia concepts:
MiP concept,
SUIT concept
MitoPedia methods:
Respirometry
MitoPedia topics: Inhibitor, Substrate and metabolite, Uncoupler
Contributed by Gnaiger E 2010-09-18, edited 2016-01-24.
SUIT protocols
- SUIT protocols have been developed for the first time on the basis of high-resolution respirometry, since their application depends critically on long-term stability of the polarographic oxygen sensor (experiments may be extended over 1 to 2 hours), highly diluted sample to avoid fast oxygen depletion in the closed chamber, and the possibility for re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitochondrial respiration media (MiR05: Gnaiger_2000_MitoInTheCold; MiR06) have been developed to maintain respiratory capacity during application of mitochondrial SUIT protocols at physiological temperature.
- Attempts are underway to develop a database of SUIT protocols: the SUIT protocol library.
