Difference between revisions of "Talk:MitoFit Proficiency Test"
From Bioblast
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== Important information for the MitoFit Proficiency Test == | == Important information for the MitoFit Proficiency Test (PT) == | ||
'''Required equipment:''' | '''Required equipment:''' | ||
Revision as of 08:47, 4 October 2018
Important information for the MitoFit Proficiency Test (PT)
Required equipment:
- Oroboros O2k, Series A-H (2004–2018)
- DatLab 7.3 (provided)
- PC/Laptop, Windows XP+
- O2k-Titration_Set with Hamilton microsyringes
Chemicals:
Experiments are to be performed:
- individually (one O2k instrument per participant)
- without any further help
- Manuals and SOPs can be used, see: O2k-technical_support_and_open_innovation
Beginner or advanced user? - Self assessment:
- Please indicate if you are a beginner or an advanced O2k user, filling out the questionnaire:
- to be added as a PDF
- Send the questionnaire to [email protected]
Time schedule
Round 01 - 22 labs invited
| Date | |
|---|---|
| 2018-10-11 | DatLab 7.3 and instructions to be sent to the participants of the study |
| 2018-10-12 to 15 | Level I PT to be performed: instrumental background oxygen flux and air calibration, using the Instrumental DLPs |
| 2018-10-15 | DatLab Files have to be sent to [email protected]
Shipping of HEK cells |
| 2018-10-17 | Arrival of shipment at customer’s site at the latest
Feedback on Level I sent to participants |
| 2018-10-17 to 26 | Level II PT to be performed: SUIT 1 / 2 technical repeats |
| 2018-10-28 | DatLab files to be sent to Oroboros for analysis (Level II) |
| 2018-10-31 | Feedback to the participants |
Scientific objective
- Together with a literature review and preliminary data from the Oroboros MitoFit laboratory, we are going to publish the test results in collaboration with the Department for Medical Statistics, Informatics and Health Economics, Medical University of Innsbruck, as part of the K-Regio Project MitoFit.
- With the participation in the MitoFit Proficiency Test, you agree on the use of experimental data collected for the test, to be used for research purposes in an anonymized way.
Time requirements
- Level 1. Air calibration and Instrumental background oxygen flux: ca. 3 hours
- Level 2. SUIT-1 or SUIT-2: ca. 3 hours, including air calibration
- Level 3. Data analysis: 1 hour
- Within the timespan of one workday, your personal proficiency is comprehensively evaluated, using your own O2k and your laboratory equipment.
Additional support
- For a comprehensive list of DatLab menus: MitoPedia DatLab
- MitoPedia - SUIT
- Flux analysis
- DatLab Protocols, DLP: List of titrations, marks, overview on the website.
