Template:Chemical SUIT-034 NADH mt D082
From Bioblast
Revision as of 13:59, 21 December 2023 by Cardoso Luiza (talk | contribs)
Step | Chemical(s) and link(s) | Comments |
---|---|---|
1D.1 | ADP (D) | 40 mM stock solution. |
2PGM | Pyruvate (P), Glutamate (G), and Malate (M) | |
3D2.5 | ADP (D) | 500 mM stock solution. |
4U | SF6847 | We do not recommend using any other uncoupler, e.g. Carbonyl cyanide m-chlorophenyl hydrazone, CCCP (U), due to the chemical background effect on fluorescence. |
5Anox | The O2 concentration in the O2k-chamber can be decreased by N2 or H2 injection to reach faster anoxia, see: Setting the oxygen concentration. | |
6Myx | Myxothiazol | We do not recommend using any other inhibitor of complex III, e.g. Antimycin A (Ama), due to the chemical background effect on fluorescence. |
7Reox | Reoxygenation can be performed by opening the chamber, see: Open chamber. |