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Difference between revisions of "Template:Current week ecosystem agenda"

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  2020 Week 37
{{Template:Science for Ukraine}}
* '''Sep-07 Mo'''  
  2024 Week 17
 
* '''Apr-22 Mo'''
:{|
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|25px|Take care]]
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| '''Why it is necessary to perform multiple H<sub>2</sub>O<sub>2</sub> calibrations when using the [[Amplex UltraRed]] (AmR) assay?'''<br>
|'''How can I evaluate the effect of one specific titration of a complex SUIT protocol?'''<br>
The sensitivity to H<sub>2</sub>O<sub>2</sub> of the Amplex UltraRed assay changes during the experiment, thus requires multiple calibrations. These sensitivity changes may be the function of: 1) experimental time, 2) changes in the optical properties of the respiration medium due to sample/chemical titration, 3) the radical scavenging capacity of the sample, and 4) the concentration of accumulating resorufin (UltroxRed in the case of Amplex UltraRed). Therefore, it is recommended to perform H<sub>2</sub>O<sub>2</sub> calibration before sample addition, after sample addition and multiple times during the experiment. <br>
Flux control efficiencies express the change in respiration after a single step, in which there is a change in a metabolic control variable.<br>
- »[[Komlodi 2018 Methods Mol Biol]]« <br>
- »'''[[Flux control efficiency]]'''«, »'''[[Metabolic control variable]]'''«<br>
 
- communicated by »[[Cardoso Luiza]]« and »[[Baglivo Eleonora]]«  
The DLPs for AmR [[SUIT]] protocols already come with multiple steps of H<sub>2</sub>O<sub>2</sub> calibrations implemented. <br>
- » [https://www.bioblast.at/index.php/SUITbrowser SUITbrowser]« <br>
- communicated by »[[Komlodi Timea]]« and »[[Cardoso Luiza]]«
|}
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* '''Sep-08 Tu'''
* '''Apr-23 Tu'''
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* '''Apr-24 We'''
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* '''Apr-25 Th'''
:{|
:{|
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
| width="100" align="center" |[[Image:O2k-Publications.jpg|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]
|'''Alternative oxidase (AOX) can prevent acute hypoxic pulmonary vasoconstriction in mouse lung'''<br>
|'''"Our results further enhance the understanding and relevance of bioenergetic function in liver ischemia and transplantation and provide the basis for further consideration of these parameters as biomarkers in LT [liver transplantation]."''' <br>
:from the O2k-Network »[[DE Giessen Weissmann N]]«, »[[ FI Tampere Dufour E]]«, »[[FI Helsinki Jacobs HT]]« and »[[DE Jena Szibor M]]«
* Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation.  [[Meszaros_2024_Transpl_Int|»Bioblast link«]]
*Sommer N, Alebrahimdehkordi N, Pak O, Knoepp F, Strielkov I, Scheibe S, Dufour E, Andjelekovic A, Sydykov A, Saraji A, Petrovic A, Quanz K, Hecker M, Kumar M, Wahl J, Kraut S, Seeger W, Schermuly RT, Ghofrani HA, Ramser K, Braun T, Jacobs HT, Weissmann N, Szibor M (2020) Bypassing mitochondrial complex III using alternative oxidase inhibits acute pulmonary oxygen sensing. Sci Adv 6:eaba0694. [[Sommer 2020 Sci Adv|»Bioblast link«]]
:from the O2k-Network »[[AT Innsbruck Schneeberger S]] and [[AT Innsbruck Oroboros]]«
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* '''Apr-26 Fr'''
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'''<big>Next Week</big>'''
2024 Week 18
 
* '''Apr-29 Mo'''
:{|
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|'''How can I update DatLab 8 to the latest version?'''<br>
If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions. <br>
- »'''[[Updating - DatLab]]'''«, »'''[https://www.oroboros.at/index.php/product/datlab/ DatLab 8]'''«<br>
- communicated by »[[Leo Elettra]]« and »[[Baglivo Eleonora]]«
|}
|}
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* '''Sep-09 We'''
* '''Apr-30 Tu'''
 
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* '''May-01 We'''  
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* '''Sep-10 Th'''
* '''May-02 Th'''  
:{|
:{|
| width="110" align="center" | [[File:MitoPedia.jpg|left|80px|MitoPedia|link=MitoPedia]]
| width="110" align="center" | [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Blue-info.png|25px|Info: improve]]
|''' What is the limitation of State 3 respiration compared to [[OXPHOS capacity]]? '''<br>
|''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>
OXPHOS capacity is defined as the maximal respiratory capacity in the OXPHOS state in the presence of kinetically-saturating concentration of ADP, P<sub>i</sub>, fuel substrates and O<sub>2</sub>. However, State 3 as defined by Chance and Williams (1955) can lead to underestimation of OXPHOS capacity and overestimation of [[excess E-P capacity]] due to kinetic limitations of the phosphorylation system. <br>
- »'''[[MiPNet21.17 BloodCellsIsolation]]'''«<br>
- »'''[[BEC_2020.1_doi10.26124bec2020-0001.v1|BEC: Mitochondrial physiology]]'''« - Table 3 and several figures. <br>
- communicated by »[[Timon-Gomez Alba]]« and »[[Baglivo Eleonora]]«
- communicated by » [[Cecatto Cristiane]] « and » [[Komlodi Timea]] «
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* '''Sep-11 Fr'''
* '''May-03 Fr'''

Revision as of 08:23, 22 April 2024

Gentle Science
Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. -  »More details
A future for Ukraine is the future of its science
2024 Week 17
  • Apr-22 Mo
MitoPedia
How can I evaluate the effect of one specific titration of a complex SUIT protocol?

Flux control efficiencies express the change in respiration after a single step, in which there is a change in a metabolic control variable.
- »Flux control efficiency«, »Metabolic control variable«
- communicated by »Cardoso Luiza« and »Baglivo Eleonora« 


  • Apr-23 Tu

  • Apr-24 We

  • Apr-25 Th
O2k-Publications in the MiPMap "Our results further enhance the understanding and relevance of bioenergetic function in liver ischemia and transplantation and provide the basis for further consideration of these parameters as biomarkers in LT [liver transplantation]."
  • Meszaros AT, Weissenbacher A, Schartner M, Egelseer-Bruendl T, Hermann M, Unterweger J, Mittelberger C, Reyer BA, Hofmann J, Zelger BG, Hautz T, Resch T, Margreiter C, Maglione M, Komlódi T, Ulmer H, Cardini B, Troppmair J, Öfner D, Gnaiger E, Schneeberger S, Oberhuber R (2024) The predictive value of graft viability and bioenergetics testing towards the outcome in liver transplantation. »Bioblast link«
from the O2k-Network »AT Innsbruck Schneeberger S and AT Innsbruck Oroboros«

  • Apr-26 Fr

Next Week

2024 Week 18
  • Apr-29 Mo
O2k-Open SupportInfo: improve How can I update DatLab 8 to the latest version?

If your computer with DatLab 8 is connected to the internet, go to “Check for updates” in the Help menu of DatLab 8, and follow the simple installation instructions.
- »Updating - DatLab«, »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«


  • Apr-30 Tu

  • May-01 We

  • May-02 Th
O2k-Open SupportInfo: improve "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented."

- »MiPNet21.17 BloodCellsIsolation«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«


  • May-03 Fr