Template:Current week ecosystem agenda
2022 Week 31
- Oroboros declares solidarity against the Russian aggression-war in Ukraine and against all aggression-wars worldwide. - »More details
- -» A future for Ukraine is the future of its science
- Aug-01 Mo
154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k Aug 1-5
O2k-checklist: Get started with an O2k-experiment
The O2k-checklist provides an overview of all essential steps for starting an O2k-experiment.
- » MiPNet20.04_O2k-checklist«
- » communicated by »Cecatto Cristiane« and »Schmitt Sabine«
- Aug-02 Tu 154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k Aug 1-5
What are the substrate-uncoupler-inhibitor titration reference protocols?
These protocols provide a common baseline for comparison of mitochondrial respiratory control in a large variety of species, tissues and cell types, mt-preparations and laboratories.
- » SUIT reference protocol«- communicated by »Cardoso Luiza HD« and »Tindle-Solomon Lisa«
- Aug-03 We 154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k Aug 1-5
O2 flux analysis with DatLab 7.4
Use the O2 analysis template:
- Aug-04 Th 154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k Aug 1-5
Why do we perform multiple H2O2 calibrations in the Amplex® UltraRed assay?
The sensitivity to H2O2 of the fluorometric signal changes during the experiment as a function of: (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, and (3) the radical scavenging capacity of the sample. Therefore, H2O2 calibrations are performed before and after sample addition, and after selected titration steps.
- »Amplex UltraRed« and »Komlodi et al BEC 2021.4«
- Aug-05 Fr 154th Oroboros O2k-Coaching Days - Advanced and NextGen-O2k Aug 1-5
Watch these videos to get started with the Q-Module
