Template:SUIT-006: Difference between revisions
From Bioblast
No edit summary |
No edit summary |
||
Line 7: | Line 7: | ||
|- | |- | ||
| 1X | | 1X<sub>SI</sub> | ||
| L | | L | ||
| ย | | ย | ||
| ย | | ย | ||
| X: combination of substrates and inhibitors. L, LEAK respiration | | X<sub>SI</sub>: combination of substrates and inhibitors. L, LEAK respiration | ||
{{Template:SUIT L n}} | {{Template:SUIT L n}} | ||
Line 20: | Line 20: | ||
| ย | | ย | ||
| ย | | ย | ||
| 1X;2D | | 1X<sub>SI</sub>;2D | ||
{{Template:SUIT OXPHOS}} | {{Template:SUIT OXPHOS}} | ||
Line 29: | Line 29: | ||
| ย | | ย | ||
| ย | | ย | ||
| 1X;2D;3U | | 1X<sub>SI</sub>;2D;3U | ||
{{Template:SUIT U*}} | {{Template:SUIT U*}} |
Revision as of 17:44, 17 January 2019
Steps and respiratory states
File:CCP for mt preparations.png
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
1XSI | L | XSI: combination of substrates and inhibitors. L, LEAK respiration | ||
2D | P | 1XSI;2D
OXPHOS capacity P (with saturating [ADP]), active OXPHOS state. | ||
3U | E | 1XSI;2D;3U
Uncoupler titration (avoiding inhibition by high uncoupler concentrations) to obtain electron transfer (ET) capacity E (noncoupled ET-state). Test for limitation of OXPHOS capacity P by the phosphorylation system (ANT, ATP synthase, phosphate transporter) relative to ET capacity E in mt-preparations: E-P control efficiency and E-L coupling efficiency. In living cells: E-R control efficiency and E-L coupling efficiency. | ||
4Ama | ROX | Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt). |
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary