Steps and respiratory states
File:1SRot;2D;3(Omy);4U;5Ama.png
Step
|
State
|
Pathway
|
Q-junction
|
Comment - Events (E) and Marks (M)
|
1Q
|
|
|
|
1Q
- To detect the redox state of the Q-pool a mimetic coenzyme Q2 is applied in suspension, which reacts both with the detecting electrode and the biological sites (CI, CII and CIII).
|
1Rot
|
|
|
|
1Rot
- Rotenone is required to detect the fully oxidized Q-pool in the presence of isolated mitochondria (or permeabilized cells) and coenzyme Q2. Rotenone can inhibit the respiration supported by endogenous substrates that remained after the isolation procedure.
|
1SRot
|
SL
|
S
|
CII
|
1SRot
|
2D
|
SP
|
S
|
CII
|
1SRot;2D
|
(3Omy)
|
ScL(Omy)
|
|
|
1SRot;2D;(3Omy)
- Omy addition is skipped in SUIT-006 O2 mt D022
- Succinate, S ( type S-pathway to Q).
- Non-phosphorylating resting state (LEAK state); LEAK-respiration, L(Omy), after blocking the ATP synthase with oligomycin.
|
4U
|
SE
|
S
|
CII
|
1SRot;2D;4U
|
5Anoxia
|
|
|
|
1SRot;2D;4U;5Anoxia
- Anoxia is a crucial step to detect the fully reduced Q-junction in the presence of coenzyme Q2 after the biological sample has consumed the O2 in the O2k-chamber for calculation of the Q redox state.
|
5Ama
|
|
|
|
1SRot;2D;4U;5Anoxia
- Rox is the residual oxygen consumption in the ROX state, due to oxidative side reactions, estimated after addition of antimycin A (inhibitor of CIII). Rox is subtracted from oxygen flux as a baseline for all respiratory states, to obtain mitochondrial respiration (mt).
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- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Β» Coupling control state
- Β» ET capacity
- Β» OXPHOS capacity
- Β» LEAK respiration
- Pathway control
- Β» Electron transfer pathway
- Β» Fatty acid oxidation pathway control state, F
- Β» NADH electron transfer-pathway state, N
- Β» Succinate pathway control state, S
- Β» NS-pathway control state, NS
- Β» Glycerophosphate pathway control state, Gp
- Β» Complex IV single step, CIV
- Β» Anaplerotic pathway control state
- Main fuel substrates
- Β» Glutamate, G
- Β» Glycerophosphate, Gp
- Β» Malate, M
- Β» Octanoylcarnitine, Oct
- Β» Pyruvate, P
- Β» Succinate, S
- Glossary
- Β» List of SUIT states
- Β» SUIT concept
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