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COST Action CA15203 (2016-2021): MitoEAGLE
Evolution-Age-Gender-Lifestyle-Environment: mitochondrial fitness mapping
Cizmarova Beata
MitoPedia topics: MitoPedia topic::EAGLE
COST: Has COST::Member COST WG1: Has COST WG1::WG1
COST WG4: Has COST WG4::WG4
| Name | Has lastname::Velika Has firstname::Beata, Has title::PhD, RNDr. |
|---|---|
| Institution | [[Has institution::::: Pavol Jozef Ε afΓ‘rik University in Kosice
Visiting scientist
Beata Velika joined Oroboros in January 2017 within the framework
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| Address | Has address::Trieda SNP 1, Has area code::040 11 |
| City | In city::KoΕ‘ice |
| State/Province | In state:: |
| Country | In country::Slovakia |
| Has mailaddress::[email protected] | |
| Weblink | Weblink:: |
| O2k-Network Lab | {{#ask:has member::Cizmarova Beata}} |
Labels:
Publications
{{#ask:was written by::Cizmarova Beata|format=table|sort=was published in year|order=desc|default=Add references to your publications
| ?was published in year=Published
| ?has title=Reference
| limit=2000
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Abstracts
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Participated at
MitoEAGLE Short-Term Scientific Mission
- Work Plan summary
- My name is Beata Velika and I work as Research specialist at the Pavol Jozef Safarik University in Kosice, Department of Medical and Clinical Biochemistry, Faculty of Medicine, Slovakia. During my study and work I specialized in fluorescence spectroscopy, and I have experience with different isolation methods, including mitochondria isolation, and measuring of their native fluorescence and respiration.
- I would like to carry out the project in cooperation with the research group at the Mitochondrial Research Laboratory of Prof. Dr. Erich Gnaiger in Innsbruck, Austria (Medical University of Innsbruck; K-Regio project MitoFit) in collaboration with the scientific company OROBOROS INSTRUMENTS Corp. The laboratory of Prof. Dr. Erich Gnaiger contains very high standard laboratory equipment, with several specialists in mitochondrial physiology field. The short-term project is focused on the optimization of SUIT (substrate-uncoupler-inhibitor titration) protocols for high-resolution respirometry of intact cryopreserved human peripheral blood mononuclear cells (PBMC). The usage of human PBMC will provide a significant stimulus for the entire field of mitochondrial medicine, because the mitochondrial function of PBMC is linked with numerous diseases and metabolic conditions in the human body, and the protocols developed during the project will lead to the standardization of the procedures, and should be used for studying mitochondria physiological and pathological mechanisms. Cryopreserved cells (stored at -80 Β°C), will be thawed in 37 Β°C water bath, dilluted in 10 ml pre-warmed 37 Β°C medium composed of DPBS + 2%FBS and centrifuged (300 g, 6 minutes). The sediment will be resuspended and counted. The suspension of cryopreserved cells will be titrated into the 2-ml chambers of the Oxygraph-2k (Oroboros Instruments, Innsbruck, Austria). We will optimize the SUIT protocols with defined substrate combinations, uncoupler and inhibitor titrations, which will be applied for the measurement of respiration of the cryopreserved cells. The results will be compared with the respiration of the fresh cells (reference). Obtained results will be presented in 10th MiPschool 2017 MitoEAGLE and MitoEAGLE Workshop WG1-4, Obergurgl AT (2017 Jul 23-30).
- Work Plan summary
MitoEAGLE Inclusiveness Target Countries - Conference Grant
Visiting scientist in the Oroboros MitoFit Laboratory
Beata Velika: Visiting scientist at the Oroboros MitoFit Laboratory
- January 08 to 19 2018
- January 15 to July 31 2017
