Difference between revisions of "High signal at zero oxygen"

Revision as of 16:20, 22 August 2023

High signal at zero oxygen

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

A high signal at zero oxygen may be observed during zero calibration (R0). The following instructions show how to further localize this technical problem.

Reference: Zero calibration, OroboPOS-Connector

High zero current test

O2k-series J and XB

O2k-series D-I and XA

1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.

2. Switch on the O2k and start DatLab.

3. Record the signal for 10 min.

4. Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".

5. Record the signal for 10 min.

6. Remove the membrane from the POS and wash the cathode and anode with distilled water.

7. Screw the POS to the POS-Connector. Set an event in DatLab called "POS".

8. Record the signal for 10 min.

9. Send us the file.

O2k-series A-C

Please start with the POS-Connector already connected to the main unit and proceed with step 5.

References:

[1] Cleaning the electrical connections

[2] MiPNet19.18B POS-service

[3] MiPNet06.03 POS-calibration-SOP

[4] MiPNet14.06 Instrumental O2 background

Example: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O₂ raw signal [V] was recorded for 10 min. The marks were set to the O₂ raw signal [V] to evaluate the zero current.

Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry

@@ Line 2: / Line 2: @@
 __TOC__
 {{MitoPedia without banner
-|abbr=''R''0
+|abbr=
-|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize this technical problem. For O2k series A-C, please start with the POS-Connector already connected to the main unit and proceed with step 5.
+|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]] (R0). The following instructions show how to further localize this technical problem.
 |info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]]
 }}
 == High zero current test==
+=== O2k-series J and XB ===
+=== O2k-series D-I and XA ===
 ::::1. Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
 ::::2. Switch on the O2k and start DatLab.
@@ Line 16: / Line 19: @@
 ::::8. Record the signal for 10 min.
 ::::9. Send us the file.
+=== O2k-series A-C ===
+:::: Please start with the POS-Connector already connected to the main unit and proceed with step 5.
 :::::References: