Difference between revisions of "High signal at zero oxygen"

Revision as of 12:21, 13 October 2023

High signal at zero oxygen

MitoPedia O2k and high-resolution respirometry: O2k-Open Support

Description

A high signal at zero oxygen may be observed during zero calibration (R0). First, check the quality of the dithionite solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.

Reference: Zero calibration, OroboPOS-Connector

Leak current test

O2k-series J and XB

Unplug the SmartPOS from the O2k-main unit.
Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water.
Switch on the O2k and start DatLab.
Record the signal for 10 min.
Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS".
Record the signal for 10 min.
Send us the file.

O2k-series D-I and XA

Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
Remove the membrane from the POS and wash the cathode and anode with distilled water.
Switch on the O2k and start DatLab.
Record the signal for 10 min.
Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
Record the signal for 10 min.
Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
Record the signal for 10 min.
Send us the file.

O2k-series A-C

Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C. Therefore, the following should be started with the O2k switched off.

Unplug the POS-Connector from the O2k-main unit and unscrew the OroboPOS from the POS-Connector. Screw the POS to the blue part of the perspex housing.
Remove the membrane from the POS and wash the cathode and anode with distilled water.
Plug the POS-Connector to the main unit.
Switch on the O2k and start DatLab.
Record the signal for 10 min.
Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
Record the signal for 10 min.
Send us the files.

References

[1] Cleaning the electrical connections

[2] MiPNet19.18B POS-service

[3] MiPNet06.03 POS-calibration-SOP

[4] MiPNet14.06 Instrumental O2 background

Example

First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O₂ raw signal [V] was recorded for 10 min. The marks were set to the O₂ raw signal [V] to evaluate the leak current.

Figure 1. Leak current test. Upper panel: Leak current is in the range of ±0.00099 V. Lower panel: Leak current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem of both, the OroboPOS-Connector, and the OroboPOS.

Keywords

Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

O2k-Procedures

» High signal at zero oxygen

» POS calibration - static

» POS calibration - dynamic

MiPNet O2k-Procedures

» POS-calibration

» O2k-Manual: POS-service

» O2k-cleaning

» O2k-Series G and DatLab 6: Calibration

General

» Oxygen, dioxygen, O₂

» Oxygen calibration - DatLab

» Oxygen solubility

» Oxygen solubility factor

» Oxygen pressure

» Concentration

» Activity

» Pressure - Pascal

» Barometric pressure

» High-resolution respirometry

» OroboPOS

» Polarographic oxygen sensor

» MitoFit Quality Control System

» Sensitivity

Other keyword lists

» Keywords: Concentration and pressure

MitoPedia O2k and high-resolution respirometry: O2k hardware, O2k-Open Support, O2k-Respirometry

@@ Line 1: / Line 1: @@
 {{Technical support}}
-__TOC__
 {{MitoPedia without banner
-|description='A ''high signal at zero oxygen''' may be observed during [[zero calibration]]. The following instructions show how to further localize and possibly solve this technical problem.
+|abbr=
+|description=A '''high signal at zero oxygen''' may be observed during [[zero calibration]] (R0). First, check the quality of the [[dithionite]] solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.
+|info= [[Zero_calibration | Zero calibration]], [[OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections | OroboPOS-Connector]]
 }}
-== High zero current ==
+__TOC__
-:::: In order to localize the problem, the following test should be performed:
+== Leak current test==
+=== O2k-series J and XB ===
+::::# Unplug the [[SmartPOS]] from the O2k-main unit.
+::::# Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water.
+::::# Switch on the O2k and start DatLab.
+::::# Record the signal for 10 min.
+::::# Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS".
+::::# Record the signal for 10 min.
+::::# Send us the file.
+=== O2k-series D-I and XA ===
+::::# Unplug the [[POS-Connector]] from the O2k-main unit and unscrew the [[OroboPOS]] from the [[POS-Connector]]. Screw the POS to the blue part of the perspex housing.
+::::# Remove the membrane from the POS and wash the cathode and anode with distilled water.
+::::# Switch on the O2k and start DatLab.
+::::# Record the signal for 10 min.
+::::# Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
+::::# Record the signal for 10 min.
+::::# Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
+::::# Record the signal for 10 min.
+::::# Send us the file.
+=== O2k-series A-C ===
+::::: Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C. Therefore, the following should be started with the O2k switched off.
+::::# Unplug the [[POS-Connector]] from the O2k-main unit and unscrew the [[OroboPOS]] from the [[POS-Connector]]. Screw the POS to the blue part of the perspex housing.
+::::# Remove the membrane from the POS and wash the cathode and anode with distilled water.
+::::# Plug the POS-Connector to the main unit.
+::::# Switch on the O2k and start DatLab.
+::::# Record the signal for 10 min.
+::::# Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
+::::# Record the signal for 10 min.
+::::# Send us the files.
-:::[[File:High zero current test 2.png|1000px]]
+=== References ===
-::::*Note: Before sending us the O2k or any spare components, please contact us via our O2k-Open Support system: [[O2k-Open Support]]
-:::::References:
 ::::::[1] [https://wiki.oroboros.at/index.php/OroboPOS-Connector_MitoPedia#Cleaning_the_electrical_connections Cleaning the electrical connections]
 ::::::[2] [[MiPNet19.18B POS-service]]
@@ Line 15: / Line 44: @@
 ::::::[4] [[MiPNet14.06 Instrumental O2 background]]
-::: '''Example:'''
+=== Example ===
-::::[[File:High zero current good example.png|600px]]
+::: First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the leak current.
-::::[[File:High zero current test bad exmaple.png|600px]]
+::::[[File:High zero current good example.png|700px]]
-::::'''Figure 1.''' High zero current test.  Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, which means that both the OroboPOS-Connector and OroboPOS have electrical problems. First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O<sub>2</sub> raw signal [V] was recorded for 10 min. The marks were set to the O<sub>2</sub> raw signal [V] to evaluate the zero current.
+::::[[File:High zero current test bad exmaple.png|700px]]
+::::'''Figure 1.''' Leak current test.  '''Upper panel:''' Leak current is in the range of ±0.00099 V. '''Lower panel:''' Leak current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem of both, the OroboPOS-Connector, and the OroboPOS.
 == Keywords ==