Horseradish peroxidase: Difference between revisions

Revision as of 15:58, 4 April 2024

high-resolution terminology - matching measurements at high-resolution

Horseradish peroxidase

Description

Horseradish peroxidase readily combines with hydrogen peroxide (H₂O₂) and the resultant [HRP-H₂O₂] complex can oxidize a wide variety of hydrogen donors.

Abbreviation: HRP

MitoPedia methods: Fluorometry

Application in HRR

HRP: Horseradish peroxidase (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C.

Preparation of 500 U/mL stock solution (dissolved in MiR05):

Weigh 3.33 mg
Dissolve in 1 mL MiR05.
Aliquot 200 µL into 500 µL Eppendorf tubes.

The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by Laszlo Tretter.

Measurement of H₂O₂ with Amplex UltraRed

Reaction

Donor + H₂O₂ → Oxidized Donor + 2 H₂O

Known inhibitors

According to Sigma-Adrich, citing ^[1]: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd²⁺, Co²⁺, Cu²⁺, Fe³⁺, Mn²⁺, Ni²⁺, and Pb²⁺ ions.

Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Amplex UltraRed

» Discussion:Amplex UltraRed

» Horseradish peroxidase

» Hydrogen peroxide

» Resorufin

General

» Extrinsic fluorophores

» MiR05-Kit

» ROS

» Sensitivity

O2k-Procedures

» Komlodi 2021 MitoFit AmR-O2 — AmplexRed, O₂ dependence, yeast

» MiPNet20.14 AmplexRed H2O2-production

» MiPNet18.05 Amplex-Mouse-heart

References

↑ Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.

[1] Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.

[1]

@@ Line 2: / Line 2: @@
 |abbr=HRP
 |description='''Horseradish peroxidase''' readily combines with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and the resultant [HRP-H<sub>2</sub>O<sub>2</sub>] complex can oxidize a wide variety of hydrogen donors.
-|info=
-|type=Enzyme
 }}
+{{MitoPedia concepts}}
 {{MitoPedia methods
 |mitopedia method=Fluorometry
 }}
+{{MitoPedia O2k and high-resolution respirometry}}
+{{MitoPedia topics}}
+__TOC__
+== Application in [[HRR]]  ==
+::: '''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P8250, store at 4 °C.
+:::: '''Preparation of 500 U/mL stock solution''' (dissolved in MiR05):
-== Application in [[HRR]]  ==
+::::# Weigh 3.33 mg
+::::# Dissolve in 1 mL MiR05.
+::::# Aliquot 200 µL into 500 µL Eppendorf tubes.
-'''HRP: Horseradish peroxidase''' (peroxidase from horseradish Type II, essentially salt-free, lyophilized powder, 150-250 units/mg solid); Sigma P 8250, store at 4 °C
+:::: The use of horseradish peroxidase type II in fluorometric measurement of peroxide production is recommended by [[Tretter L|Laszlo Tretter]].
+:::: [[Amplex_UltraRed|Measurement of H<sub>2</sub>O<sub>2</sub> with Amplex UltraRed]]
 == Reaction ==
-Donor + H<sub>2</sub>O<sub>2</sub> → Oxidized Donor + 2 H<sub>2</sub>O
+:::: Donor + H<sub>2</sub>O<sub>2</sub> → Oxidized Donor + 2 H<sub>2</sub>O
+== Known inhibitors ==
+:::: According to [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/1/p8250dat.Par.0001.File.tmp/p8250dat.pdf Sigma-Adrich], citing <ref>Zollner H (1993) Handbook of Enzyme Inhibitors, 2nd ed, Part A:367-8.</ref>: sodium azide, cyanide, L-cystine,dichromate, ethylenethiourea, hydroxylamine, sulfide, vanadate, p-aminobenzoic acid, and Cd<sup>2+</sup>, Co<sup>2+</sup>, Cu<sup>2+</sup>, Fe<sup>3+</sup>, Mn<sup>2+</sup>, Ni<sup>2+</sup>, and Pb<sup>2+</sup> ions.
+{{Keywords: H2O2}}
-== Comment ==
+== References ==
-The use of horseradish peroxidase Typ II in fluorometric measurement of peroxide production was recommended by [[Tretter L|Laszlo Tretter]].
+<references/>