MiPNet06.03 POS-calibration-SOP
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Polarographic oxygen sensors: calibration, accuracy and quality control SOP. ยปBioblast pdfยซ |
ยป Versions
OROBOROS (2015-05-10) Mitochondr Physiol Network
Abstract: Gnaiger E (2015) Polarographic oxygen sensors: calibration, accuracy and quality control SOP. Mitochondr Physiol Network 06.03(13):1-8.
High-resolution respirometry critically depends on accurate calibration of the polarographic oxygen sensors which respond to partial oxygen pressure. Expressing the oxygen signal in terms of partial pressure has several advantages, but respiration is expressed in molar units related to biochemical stoichiometries. Conversion of oxygen partial pressure to oxygen concentration requires accurate information on oxygen solubilities in experimental incubation media. Absolute errors up to 10% are commonly found in the established literature. The oxygen solubility of mitochondrial respiration medium MiR06 relative to pure water (oxygen solubility factor, FM) is 0.92, accurately determined (for MiR05, i.e. MiR06 without catalase; MiPNet14.13) at 37 ยฐC and 30 ยฐC. At air saturation at standard barometric pressure (100 kPa) and 37 ยฐC, the partial oxygen pressure is 19.63 kPa, and the oxygen concentration is 190.7 ยตM in MiR06. A clean and ready-to-use O2k-Chamber is a requirement for saving time and quickly calibrating the polarographic oxygen sensors.
- ยป Product: OroboPOS, Oxygraph-2k, O2k-Catalogue
โข O2k-Network Lab: AT_Innsbruck_OROBOROS
Labels: MiParea: Instruments;methods
HRR: Oxygraph-2k, Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property.
O2k-SOP
Further details
- O2k-Manual: ยปMiPNet19.18D_O2k-Calibrationยซ
- Next step of instrumental quality control: ยปMiPNet14.06 InstrumentalBackgroundยซ
- Forstner H, Gnaiger E (1983) Calculation of equilibrium oxygen concentration. In: Polarographic Oxygen Sensors. Aquatic and Physiological Applications. Gnaiger E, Forstner H (eds), Springer, Berlin, Heidelberg, New York:321-33. ยปBioblast Accessยซ
Response time of the POS
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
The response time of the Polarographic oxygen sensor is assessed in the Stirrer test that is part of a full Sensor test. A quantitative interpretation of the stirrer test will yield the time constant of the sensor. Such a quantitative calculation is indeed necessary for kinetic work. However, to access the suitability of a sensor for standard steady state work each user will soon gain the necessary experience to judge the outcome of a stirrer test.
- >> Example: Download here.
For how to improve the response time of a POS, see Slow responding sensor.
The response time of the sensor is just one of several factors determining the time resolution.
This article is cited in
- Rodriguez JV, Pizarro MD, Scandizzi AL, Guibert EE, Almada LL, Mamprin ME (2008) Construction and performance of a minibioreactor suitable as experimental bioartificial liver. Artif Organs 32:323-8. ยปPubMed 18370948ยซ