|
Β |
(40 intermediate revisions by 10 users not shown) |
Line 1: |
Line 1: |
| {{Mainpage OROBOROS}} | | {{OROBOROS header page name}} |
| {{Publication | | {{Publication |
| |title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|OROBOROS]] Gnaiger E, Fasching M (2014) Oxygraph-2k and O2k-MultiSensor system: specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10. | | |title=[[Image:Logo OROBOROS INSTRUMENTS.jpg|right|60px|link=http://www.oroboros.at|Oroboros ]] O2k-specifications for respirometry and comprehensive OXPHOS analysis. |
| |info=[[Media:MiPNet18.10 O2kvsMultiwell.pdf|'''Open Access-MiPNet18.10.pdf''']] ; [http://www.bioblast.at/index.php/File:MiPNet18.10_O2kvsMultiwell.pdf Versions] | | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/d/db/MiPNet18.10_O2k_specifications.pdf|Bioblast pdf]] Β» [http://www.bioblast.at/index.php/File:MiPNet18.10_O2k_specifications.pdf Versions] |
| |authors=OROBOROS | | |authors=Oroboros |
| |year=2014 | | |year=2024-03-26 |
| |journal=Mitochondr Physiol Network | | |journal=Mitochondr Physiol Network |
| |keywords=O2k-Specifications | | |abstract=Gnaiger E (2024) O2k-Specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(10):1-8. |
| |mipnetlab=AT Innsbruck OROBOROS | | {{MiPNet pdf page linking to MitoPedia}} |
| | |mipnetlab=AT Innsbruck Oroboros |
| }} | | }} |
| | == Further information == |
| | ::::Β» [[MiPNet06.05 Test Experiments on O2k-Specifications]] |
| | ::::Β» [[Comparison of respirometric methods]] |
| | <br /> |
| | '''Acknowledgements''' |
| | [[File:Template NextGen-O2k.jpg|left|400px|link=NextGen-O2k]] |
| | <br/> |
| | <br/> |
| | <br/> |
| | <br/> |
| | <br/> |
| | |
| {{Labeling | | {{Labeling |
| |area=Respiration, Instruments;methods | | |area=Respiration, Instruments;methods |
| |organism=Human | | |organism=Human |
| |model cell lines=Fibroblast | | |tissues=Fibroblast |
| |preparations=Intact cells, Permeabilized cells, Permeabilized tissue, Homogenate, Isolated Mitochondria, SMP | | |preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Intact cells |
| |instruments=Oxygraph-2k, TIP2k, Fluorometry, pH, NO, TPP, Ca, Theory | | |instruments=Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory |
| | |additional=Comparison of respirometric methods, MitoFit2022QC |
| }} | | }} |
| >> '''''More details on O2k-Specifications?'' [http://www.oroboros.at/?O2k-Specifications Consult OROBOROS]'''
| |
|
| |
| == Discussion ==
| |
| Scientists who have experience with both instruments are invited to extend the discussion:
| |
|
| |
| ::>> [[Talk:MiPNet18.10_O2k_versus_multiwell]]
| |
| ::>> [[Talk:Rogers 2011 PLoS One|XFe for isolated mitochondria - a critical discussion]]
| |
|
| |
| == Selected related links ==
| |
|
| |
| ::>> [http://www.oroboros.at/?low-o2k-running-costs Low running costs for the OROBOROS O2k]
| |
| ::>> [http://www.oroboros.at/?Power-O2k Power-O2k]
| |
| :: >> [[O2k-Publications:_Specific_topics#Tissues_and_cell_types]]
| |
|
| |
| == Conflict of interest ==
| |
| :: [[Gnaiger E|Erich Gnaiger]] is founder and CEO of OROBOROS INSTRUMENTS, and responsible for the development of the Oxygraph-2k in collaboration with the O2k-Team and our partners. A series of methodological publications is the result of positive feedback between his work as faculty staff of the Medical University of Innsbruck, his company initiatives and numerous international cooperations.
| |
|
| |
| == Selected references ==
| |
|
| |
| ::* [[Gnaiger 1995 J Bioenerg Biomembr|Gnaiger E, Steinlechner-Maran R, MΓ©ndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. J Bioenerg Biomembr 27: 583-596.]]
| |
|
| |
| ::* [[Steinlechner-Maran 1996 Am J Physiol Cell Physiol|Steinlechner-Maran R, Eberl T, Kunc M, Margreiter R, Gnaiger E (1996) Oxygen dependence of respiration in coupled and uncoupled endothelial cells. Am J Physiol Cell Physiol 271: C2053-C2061.]]
| |
|
| |
| ::* [[Gnaiger 2000 Proc Natl Acad Sci U S A|Gnaiger E, MΓ©ndez G, Hand SC (2000) High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia. Proc Natl Acad Sci U S A 97: 11080-11085.]]
| |
|
| |
| ::* [[Gnaiger 2001 Respir Physiol|Gnaiger E (2001) Bioenergetics at low oxygen: dependence of respiration and phosphorylation on oxygen and adenosine diphosphate supply. Respir Physiol 128: 277-297.]]
| |
|
| |
| ::* [[Huetter 2004 Biochem J|HΓΌtter E, Renner K, Pfister G, StΓΆckl P, Jansen-DΓΌrr P, Gnaiger E (2004) Senescence-associated changes in respiration and oxidative phosphorylation in primary human fibroblasts. Biochem J 380: 919-928.]]
| |
|
| |
| ::* [[Kuznetsov 2004 Am J Physiol Heart Circ Physiol|Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Mark W, Steurer W, Saks V, Usson Y, Margreiter R, Gnaiger E (2004) Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am J Physiol Heart Circ Physiol 286: H1633βH1641.]]
| |
|
| |
| ::* [[Gnaiger 2008 POS|Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley: 327-352.]]
| |
|
| |
| ::* [[Scandurra 2010 Adv Exp Med Biol|Scandurra FM, Gnaiger E (2010) Cell respiration under hypoxia: Facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 662: 7-25.]]
| |
|
| |
| ::* [[Aguirre 2010 Biochim Biophys Acta|Aguirre E, RodrΓguez-JuΓ‘rez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797: 557-565.]]
| |
|
| |
| ::* [[Votion 2012 PLoS One|Votion DM, Gnaiger E, Lemieux H, Mouithys-Mickalad A, Serteyn D (2012) Physical fitness and mitochondrial respiratory capacity in horse skeletal muscle. PLoS One 7: e34890.]]
| |
|
| |
| ::* [[Pesta 2012 Methods Mol Biol|Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810: 25-58.]]
| |
|
| |
| ::* [[Gnaiger 2012 MitoPathways|Gnaiger E (2012) Mitochondrial Pathways and Respiratory Control. An Introduction to OXPHOS Analysis. Mitochondr Physiol Network 17.18. OROBOROS MiPNet Publications, Innsbruck: 64 pp.]]
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
| {{MitoPedia methods
| |
|
| |
| |mitopedia method=Respirometry
| |
|
| |
| |type=Respiration
| |
|
| |
| }}
| |
|
| |
| {{MitoPedia topics|type=Respiration
| |
|
| |
| }}
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
| |
| [[Category:OroboPedia]]
| |
MiPNet18.10 O2k-Specifications
O2k-specifications for respirometry and comprehensive OXPHOS analysis.
|
Β» Β» Versions
Oroboros (2024-03-26) Mitochondr Physiol Network
Abstract: Gnaiger E (2024) O2k-Specifications for respirometry and comprehensive OXPHOS analysis. Mitochondr Physiol Network 18.10(10):1-8.
- Open the pdf document above.
β’ O2k-Network Lab: AT Innsbruck Oroboros
Further information
- Β» MiPNet06.05 Test Experiments on O2k-Specifications
- Β» Comparison of respirometric methods
Acknowledgements
Labels: MiParea: Respiration, Instruments;methods
Organism: Human
Tissue;cell: Fibroblast
Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, SMP, Intact cells
HRR: Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, Theory
Comparison of respirometric methods, MitoFit2022QC