MiPNet19.05 IOC90 Bolzano IT: Difference between revisions
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== Conference == | == Conference == | ||
* [http://www.ismm2014.org/Pages/default.aspx ISMM2014] World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31 | ::::* [http://www.ismm2014.org/Pages/default.aspx ISMM2014] World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31 | ||
== O2k-Workshop == | == O2k-Workshop == | ||
:: TUESDAY 2014-05-27 / 14.00 - 17.30 | ::: TUESDAY 2014-05-27 / 14.00 - 17.30 | ||
:: Seminar room 8 | ::: Seminar room 8 | ||
The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H<sub>2</sub>O<sub>2</sub> production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’. | :::: The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H<sub>2</sub>O<sub>2</sub> production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’. | ||
::::» ''More details? Consult'' [[Hypothermia-Network: Mitochondria in the Cold]]. | |||
=== Introduction === | === Introduction === | ||
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[[Fleischmann Sandra]]; Administrative assistant; | [[Fleischmann Sandra]]; Administrative assistant; | ||
[[Laner Verena]]; Project manager and scientific assistant | [[Laner Verena]]; Project manager and scientific assistant | ||
* [[OROBOROS INSTRUMENTS]] - General introduction into the [[Oxygraph-2k]] and [[high-resolution respirometry]], O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module [[Titration-Injection microPump]]). | ::::* [[OROBOROS INSTRUMENTS]] - General introduction into the [[Oxygraph-2k]] and [[high-resolution respirometry]], O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module [[Titration-Injection microPump]]). | ||
=== Demo experiment === | === Demo experiment === | ||
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: [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link=Gnaiger_Erich|Erich Gnaiger, Greenland]] [[Image:Boushel Robert C.JPG|100px|link=Boushel_RC|Robert Boushel]] [[Image:Chicco Headshot.jpg|75px|link=Chicco_Adam_J|Adam Chicco]] [[Image:Murray Andrew J.jpg|130px|link=Murray_Andrew_J|Andrew Murray]] [[Image:Horscroft James A.jpg|85px|link=Horscroft_James_A|James Horscroft]] | : [[Image:Erich Gnaiger Wolstenhjolm Fjord March 2004.jpg|90px|link=Gnaiger_Erich|Erich Gnaiger, Greenland]] [[Image:Boushel Robert C.JPG|100px|link=Boushel_RC|Robert Boushel]] [[Image:Chicco Headshot.jpg|75px|link=Chicco_Adam_J|Adam Chicco]] [[Image:Murray Andrew J.jpg|130px|link=Murray_Andrew_J|Andrew Murray]] [[Image:Horscroft James A.jpg|85px|link=Horscroft_James_A|James Horscroft]] | ||
Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | :::: Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function. | ||
* [[Gnaiger Erich]], AT - [[Boushel_RC]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK | ::::* [[Gnaiger Erich]], AT - [[Boushel_RC]], SE - [[Chicco Adam J]], US - [[Murray Andrew J]], UK - [[Horscroft JA]], UK | ||
=== Round table: Hypothermia and mitochondrial function - perspectives === | === Round table: Hypothermia and mitochondrial function - perspectives === | ||
'''16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function''' | '''16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function''' | ||
Keynote statements by | ::: Keynote statements by | ||
* Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants | ::::* Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants | ||
[[File:Ojuka Edward-Adam Chicco-Erich Gnaiger ISMM2014 2445s.JPG|right|240px|Edward Ojuka, Adam Chicco, Erich Gnaiger; ISMM2014 Bolzano]] | [[File:Ojuka Edward-Adam Chicco-Erich Gnaiger ISMM2014 2445s.JPG|right|240px|Edward Ojuka, Adam Chicco, Erich Gnaiger; ISMM2014 Bolzano]] | ||
=== Participants === | === Participants === | ||
* Harald Engan (Mid Sweden University) | :::* Harald Engan (Mid Sweden University) | ||
* [[Pichler I |Irene Pichler]] (EURAC Bolzano/Bozen - Center for Biomedicine) | :::* [[Pichler I |Irene Pichler]] (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
* [[Benage L |Lindsay Benage]] (Colorado State University) | :::* [[Benage L |Lindsay Benage]] (Colorado State University) | ||
* Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine) | :::* Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
* Mark Olfert (West Virginia University) | :::* Mark Olfert (West Virginia University) | ||
* Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin) | :::* Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin) | ||
* Chris Müller (LMU München) | :::* Chris Müller (LMU München) | ||
* Vittore Verrati (Chieti University) | :::* Vittore Verrati (Chieti University) | ||
* Marika Falla (Ospedale di Bolzano, Neurologia) | :::* Marika Falla (Ospedale di Bolzano, Neurologia) | ||
* Paolo Laveder (University of Padova, Italy, Dep of Biology) | :::* Paolo Laveder (University of Padova, Italy, Dep of Biology) | ||
* [[Martin D |Daniel Martin]] (University College London) | :::* [[Martin D |Daniel Martin]] (University College London) | ||
* [[Ojuka E|Edward Ojuka]] (University of Cape Town, South Africa) | :::* [[Ojuka E|Edward Ojuka]] (University of Cape Town, South Africa) | ||
=== Guest tutors and invited experts === | === Guest tutors and invited experts === | ||
* [[Boushel_RC|Robert Boushel]] (The Swedish School of Sport and Health Sciences) | :::* [[Boushel_RC|Robert Boushel]] (The Swedish School of Sport and Health Sciences) | ||
* [[Chicco Adam J|Adam Chicco]] (Colorado State University, Department of Health and Exercise Science) | :::* [[Chicco Adam J|Adam Chicco]] (Colorado State University, Department of Health and Exercise Science) | ||
* [[Murray Andrew J|Andrew Murray]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | :::* [[Murray Andrew J|Andrew Murray]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | ||
* [[Horscroft JA|James Horscroft]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | :::* [[Horscroft JA|James Horscroft]] (University of Cambrigde - Dept of Physiology, Development & Neuroscience) | ||
* [[Malacrida S|Sandro Malacrida]] (EURAC Bolzano/Bozen - Center for Biomedicine) | :::* [[Malacrida S|Sandro Malacrida]] (EURAC Bolzano/Bozen - Center for Biomedicine) | ||
=== Registration === | === Registration === | ||
* Visit us at the [[OROBOROS INSTRUMENTS]] exhibition booth from Monday to Wednesday, May 26 to 28. | ::::* Visit us at the [[OROBOROS INSTRUMENTS]] exhibition booth from Monday to Wednesday, May 26 to 28. | ||
* Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants. | ::::* Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants. | ||
== Recommended reading == | == Recommended reading == | ||
* [[O2k-Publications: Hypoxia]] | ::::* [[O2k-Publications: Hypoxia]] | ||
:: Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. [[Clanton 2013 Compr Physiol |»Abstract]] | ::::* Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. [[Clanton 2013 Compr Physiol |»Abstract]] | ||
* [[O2k-Publications: Oxygen kinetics]] | ::::* [[O2k-Publications: Oxygen kinetics]] | ||
* [[O2k-Publications: Temperature]] | ::::* [[O2k-Publications: Temperature]] | ||
| Line 108: | Line 109: | ||
=== SUIT protocols for high-resolution respirometry === | === SUIT protocols for high-resolution respirometry === | ||
[[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] | [[Image:O2k-Publications.jpg|left|50px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] | ||
Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »[[Pesta 2012 Methods Mol Biol |Bioblast Access]] | :::: Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »[[Pesta 2012 Methods Mol Biol |Bioblast Access]] | ||
* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »[[Gnaiger_2008_POS |Bioblast Access]] | ::::* Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »[[Gnaiger_2008_POS |Bioblast Access]] | ||
=== HRR and O2k-Fluorometry === | === HRR and O2k-Fluorometry === | ||
::::» [[MiPNet17.05 O2k-Fluo LED2-Module|Manual: MiPNet17.05 O2k-Fluo LED2-Module]] | |||
* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »[[MiPNet18.05 Amplex-Mouse-heart |Open Access]] | ::::* Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H<sub>2</sub>O<sub>2</Sub> production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »[[MiPNet18.05 Amplex-Mouse-heart |Open Access]] | ||
::::» [[O2k-Publications: O2k-Fluorometry|O2k-Fluorometry]]''' | |||
=== Mitochondrial pathways === | === Mitochondrial pathways === | ||
[[Image:O2k-Protocols.jpg|left|40px|link=O2k-Protocols|O2k-Protocols]] | [[Image:O2k-Protocols.jpg|left|40px|link=O2k-Protocols|O2k-Protocols]] | ||
Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. »[[Gnaiger 2014 MitoPathways |Open Access]] - '''''handout to O2k-Workshop participants'''''. | ::::* Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. » [[Gnaiger 2014 MitoPathways |Open Access]] - '''''handout to O2k-Workshop participants'''''. | ||
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[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] | ||
O2k-Workshops are listed as [[MitoGlobal Events]]. | O2k-Workshops are listed as [[MitoGlobal Events]]. | ||
* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''. | ::::* This workshop is a component of the K-Regio project ''[[MitoCom_O2k-Fluorometer|MitoCom]]''. | ||
[[Category:O2k-Workshops]] | [[Category:O2k-Workshops]] | ||
Revision as of 11:24, 20 May 2016
MiPNet19.05 IOC90 Bolzano IT
 Bolzano IT, 2014 May 27. Applied Workshop - High-resolution respirometry and mitochondrial function under hypoxia: overview, demo experiment and discussion. IOC90 at ISMM2014. |
» 
OROBOROS (2014-05-27) Mitochondr Physiol Network
Abstract:
90th OROBOROS O2k-Workshop on high-resolution respirometry and O2k-Fluorometry.
• O2k-Network Lab: AT Innsbruck OROBOROS
Labels:
Stress:Ischemia-reperfusion, Temperature
HRR: Oxygraph-2k
ORO, IOC, 2014, MitoCom
Conference
- ISMM2014 World Congress on High Altitude Medicine and Physiology, Bolzano IT, 2014 May 25-31
O2k-Workshop
- TUESDAY 2014-05-27 / 14.00 - 17.30
- Seminar room 8
- The 90th O2k-Workshop introduces high-resolution respirometry and O2k-Fluorometry and presents a real-time demoexperiment on mitochondrial respiration and H2O2 production under hypoxia. Several participants of the Mitochondrial Session (Monday) will discuss general and specific methodological aspects of applications of the OROBOROS Oxygraph-2k in the context of hypoxia and hypothermia. A final round table discussion is aimed at extending a network on research on ‘Clinical Hypothermia and Mitochondrial Function’.
- » More details? Consult Hypothermia-Network: Mitochondria in the Cold.
Introduction
14:00 – 14:45 Introduction: high-resolution respirometry and mt-function in extreme environments
Gnaiger Erich, CEO;
Fontana-Ayoub Mona; Biomedical assistant;
Fleischmann Sandra; Administrative assistant;
Laner Verena; Project manager and scientific assistant
- OROBOROS INSTRUMENTS - General introduction into the Oxygraph-2k and high-resolution respirometry, O2k-Demo experiment on mitochondrial respiratory function under hypoxic steady-state (O2k with module Titration-Injection microPump).
Demo experiment
14:45 – 15:30 Demo experiment: mitochondrial respiration and H2O2 production under hypoxia
15:30 – 16:00 Coffee break
Round table: Hypoxia and mitochondrial function - practical experience
16:00 – 16.45 Round table discussion: Experience with mitochondrial respiration taking the Oxygraph-2k to >4,000 m
- Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
- Gnaiger Erich, AT - Boushel_RC, SE - Chicco Adam J, US - Murray Andrew J, UK - Horscroft JA, UK
- Participants at Mitochondrial Session (Monday, 14:00-17:30, general conference programme) present methodological perspectives of their work on mitochondrial respiratory function.
Round table: Hypothermia and mitochondrial function - perspectives
16:45 – 17:00 Round table discussion: Clinical Hypothermia and Mitochondrial Function
- Keynote statements by
- Strapazzon Giacomo, IT - Malacrida Sandro, IT - and ad hoc participants
- Keynote statements by
Participants
- Harald Engan (Mid Sweden University)
- Irene Pichler (EURAC Bolzano/Bozen - Center for Biomedicine)
- Lindsay Benage (Colorado State University)
- Alessandra Zanon (EURAC Bolzano/Bozen - Center for Biomedicine)
- Mark Olfert (West Virginia University)
- Petra Platen (Prof Dr Ruhr University Bochum, Sportmedizin)
- Chris Müller (LMU München)
- Vittore Verrati (Chieti University)
- Marika Falla (Ospedale di Bolzano, Neurologia)
- Paolo Laveder (University of Padova, Italy, Dep of Biology)
- Daniel Martin (University College London)
- Edward Ojuka (University of Cape Town, South Africa)
Guest tutors and invited experts
- Robert Boushel (The Swedish School of Sport and Health Sciences)
- Adam Chicco (Colorado State University, Department of Health and Exercise Science)
- Andrew Murray (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- James Horscroft (University of Cambrigde - Dept of Physiology, Development & Neuroscience)
- Sandro Malacrida (EURAC Bolzano/Bozen - Center for Biomedicine)
Registration
- Visit us at the OROBOROS INSTRUMENTS exhibition booth from Monday to Wednesday, May 26 to 28.
- Here you can register for the OROBOROS O2k-Workshop until Tuesday morning, May 27. Free registration for ISMM participants.
Recommended reading
- O2k-Publications: Hypoxia
- Clanton TL, Hogan MC, Gladden LB (2013) Regulation of cellular gas exchange, oxygen sensing, and metabolic control. Compr Physiol 3:1135-90. »Abstract
- O2k-Publications: Oxygen kinetics
- O2k-Publications: Temperature
SUIT protocols for high-resolution respirometry
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
- Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial Dysfunction in Drug-Induced Toxicity (Dykens JA, Will Y, eds) John Wiley:327-52. »Bioblast Access
- Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibres from small biopisies of human muscle. Methods Mol Biol 810:25-58. »Bioblast Access
HRR and O2k-Fluorometry
- » Manual: MiPNet17.05 O2k-Fluo LED2-Module
- Eigentler A, Fontana-Ayoub M, Gnaiger E (2013) O2k-Fluorometry: HRR and H2O2 production in mouse cardiac tissue homogenate. Mitochondr Physiol Network 18.05(01):1-6. »Open Access
- » O2k-Fluorometry
- » Manual: MiPNet17.05 O2k-Fluo LED2-Module
Mitochondrial pathways
- Gnaiger E (2014) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 4th ed. Mitochondr Physiol Network 19.12. OROBOROS MiPNet Publications, Innsbruck:80 pp. » Open Access - handout to O2k-Workshop participants.
O2k-Workshops are listed as MitoGlobal Events.
- This workshop is a component of the K-Regio project MitoCom.
