MiPNet28.03 IOC157 Los Angeles US: Difference between revisions
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|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | |abstract=[[Image:O2k-Workshops.png|left|130px|link=O2k-Workshops]] | ||
'''Oroboros O2k-Workshop on high-resolution respirometry (HRR)''' in cooperation with [[Shirihai Orian]]. Los Angeles, US, 2023 February 14-16. | '''Oroboros O2k-Workshop on high-resolution respirometry (HRR)''' in cooperation with [[US CA Los Angeles_Shirihai O |Shirihai Orian]]. Los Angeles, US, 2023 February 14-16. | ||
|mipnetlab=AT_Innsbruck_Oroboros | |mipnetlab=US CA Los Angeles_Shirihai O, AT_Innsbruck_Oroboros ย | ||
}} | }} | ||
[[Image:O2k-Workshops.png|right|150px|link=http://wiki.oroboros.at/index.php/Oroboros_Events#Next_O2k-Workshops|O2k-Workshops]] | [[Image:O2k-Workshops.png|right|150px|link=http://wiki.oroboros.at/index.php/Oroboros_Events#Next_O2k-Workshops|O2k-Workshops]] | ||
__TOC__ ย | __TOC__ ย | ||
== General information == | |||
[[File:IOC157 Los Angeles US team.JPG|500px|right|The IOC157 team]] | |||
[[File:IOC157 09.JPG|500px|right|IOC157 Lunch]] | |||
:::: '''Oroboros Instruments (Innsbruck, Austria) - 157<sup>th</sup> O2k-Workshop, UCLA''' | |||
:::: Cutting-edge innovations of '''high-resolution respirometry (HRR)''' are presented by ''Erich Gnaiger'', introducing the '''Oroboros NextGen-O2k''' [1] in parallel to hands-on training by ''Sabine Schmitt''. | |||
:::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. Assays for mt-membrane potential, ROS production, ATP production, and calcium uptake are integrated in each chamber simultaneously with HRR. Modules for monitoring '''Q- and NADH-redox states and PhotoBiology''' with the NextGen-O2k open new windows for bioenergetic investigations. | |||
::::# [https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ NextGen-O2k all-in-one]ย | |||
::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions. | |||
::::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | |||
== | == Venue and organizers == ย | ||
ย | |||
:::: UCLA Campus | :::: UCLA Campus | ||
:::: 650 Charles E Young Drive South Suite | :::: 650 Charles E Young Drive South Suite | ||
Line 24: | Line 30: | ||
:::: Los Angeles, CA 90095, USA | :::: Los Angeles, CA 90095, USA | ||
:::: [https://wiki.oroboros.at/images/5/55/Map_CHS_Hevener_Laboratory.pdf Map] | :::: [https://wiki.oroboros.at/images/5/55/Map_CHS_Hevener_Laboratory.pdf Map] | ||
ย | <gallery mode=default perrow=4 widths="140px" heights="150px"> | ||
File:Profile-icon-9.png|'''[[Hevener Andrea|Prof. Andrea Hevener]]''', University of California, Los Angeles, US | |||
<gallery mode=default perrow= | File:Shirihai_O.jpg|'''[[Shirihai Orian|Prof. Orian Shirihai]]''', University of California, Los Angeles, US | ||
File:Profile-icon-9.png|'''[[Hevener Andrea| | File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD, Oroboros Instruments - CEO, ''Innovation Alchemist'' | ||
File:Shirihai_O.jpg|'''[[Shirihai Orian| | File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD, Oroboros Instruments - ''Mitochondrial Detective'' | ||
</gallery> ย | </gallery> ย | ||
== Program == | == Program == | ||
:::* Tuesday, February 14<sup>th</sup> CHS 27-200c - [https://wiki.oroboros.at/images/a/a5/2023-02-14_Oroboros_Instruments_O2k-Workshop_UCLA.pdf '''''downlodad pdf'''''] | |||
ย | :::: 10:00-10:50am Principles of high-resolution respirometry HRR | ||
:::: 11:00-11:50am The NextGen-O2k for Q- and NADH- redox biology | |||
:::: 1:00-1:50pm Application of fluorescence probes with HRR for mt-phenotyping | |||
:::: 4:00-5:00pm SuperMito Seminar | |||
:::::: '''Erich Gnaiger: mitObesity - body mass excess and decline of mitochondrial fitness: from muscle to brain''' | |||
:::::: Obesity is defined as accumulation of excess fat tissue mass. Compromised mitochondrial fitness across metabolically active organs provides a mechanistic connection between obesity and comorbidities: diabetes, cardiovascular and neurodegenerative diseases, and various types of cancer bound to redox imbalance, inflammation, oxidative stress, and insulin resistance. | |||
ย | |||
== Participants == | == Participants == | ||
[[File:IOC157 05.JPG|500px|right|IOC157 Sabine introduces Oxia]] | |||
[[File:IOC157 08.JPG|500px|right|IOC157 Gas injection from Oxia]] | |||
{| class="wikitable" | {| class="wikitable" | ||
|- | |- | ||
Line 51: | Line 58: | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Souvenir Rhonda ]] | | [[Souvenir Rhonda ]] | ||
| | | [[US CA Los Angeles_Shirihai O]], UCLA, US | ||
|- | |- | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Stiles Linsey]] | | [[Stiles Linsey]] | ||
| | | [[US CA Los Angeles_Shirihai O]], UCLA, US | ||
|- | |- | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Tran Peter]] | | [[Tran Peter]] | ||
| | | [[US CA Los Angeles_Shirihai O]], UCLA, US | ||
|- | |- | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Zhang Yuan]] | | [[Zhang Yuan]] | ||
| | | [[US CA Los Angeles_Shirihai O]], UCLA, US | ||
|- | |- | ||
| [[File:Profile-icon-9.png|70px|Participant]] | | [[File:Profile-icon-9.png|70px|Participant]] | ||
| [[Zhou Zhenqi]] | | [[Zhou Zhenqi]] | ||
| | | [[US CA Los Angeles_Shirihai O]], UCLA, US | ||
|- | |- | ||
|} | |} | ||
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== IOC recommended reading == | == IOC recommended reading == | ||
::::ยป '''[[IOC recommended reading]]''' | ::::ยป '''[[IOC recommended reading]]''' | ||
[[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] ย | [[Image:MitoGlobal.jpg|right|80px|link=MitoGlobal|MitoGlobal]] ย | ||
ย O2k-Workshops are listed as [[MitoGlobal Events]].' | ย O2k-Workshops are listed as [[MitoGlobal Events]].' | ||
{{Labeling | {{Labeling | ||
|instruments=Oxygraph-2k | |instruments=Oxygraph-2k | ||
|additional=ORO, IOC, O2k-Network, 2023, MitoGlobal | |additional=ORO, IOC, O2k-Network, 2023, MitoGlobal | ||
}} | }} | ||
Latest revision as of 18:48, 1 March 2023
MiPNet28.03 IOC157 Los Angeles US
Los Angeles, CA US, 2023 Feb 14-16. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC157. |
Oroboros (2023-02-14) Mitochondr Physiol Network
Abstract:
Oroboros O2k-Workshop on high-resolution respirometry (HRR) in cooperation with Shirihai Orian. Los Angeles, US, 2023 February 14-16.
โข O2k-Network Lab: US CA Los Angeles_Shirihai O, AT_Innsbruck_Oroboros
General information
- Oroboros Instruments (Innsbruck, Austria) - 157th O2k-Workshop, UCLA
- Cutting-edge innovations of high-resolution respirometry (HRR) are presented by Erich Gnaiger, introducing the Oroboros NextGen-O2k [1] in parallel to hands-on training by Sabine Schmitt.
- HRR targets reproducibility and flexibility of substrate-uncoupler-inhibitor titration protocols [2] for investigating mitochondrial pathway and coupling control [3]. Assays for mt-membrane potential, ROS production, ATP production, and calcium uptake are integrated in each chamber simultaneously with HRR. Modules for monitoring Q- and NADH-redox states and PhotoBiology with the NextGen-O2k open new windows for bioenergetic investigations.
- NextGen-O2k all-in-one
- SUIT protocols - The SUITbrowser helps you find the best SUIT protocol for your specific research questions.
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Venue and organizers
- UCLA Campus
- 650 Charles E Young Drive South Suite
- Center for Health Sciences, Suite 34-115
- Los Angeles, CA 90095, USA
- Map
Prof. Andrea Hevener, University of California, Los Angeles, US
Prof. Orian Shirihai, University of California, Los Angeles, US
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Sabine Schmitt, PhD, Oroboros Instruments - Mitochondrial Detective
Program
- Tuesday, February 14th CHS 27-200c - downlodad pdf
- 10:00-10:50am Principles of high-resolution respirometry HRR
- 11:00-11:50am The NextGen-O2k for Q- and NADH- redox biology
- 1:00-1:50pm Application of fluorescence probes with HRR for mt-phenotyping
- 4:00-5:00pm SuperMito Seminar
- Erich Gnaiger: mitObesity - body mass excess and decline of mitochondrial fitness: from muscle to brain
- Obesity is defined as accumulation of excess fat tissue mass. Compromised mitochondrial fitness across metabolically active organs provides a mechanistic connection between obesity and comorbidities: diabetes, cardiovascular and neurodegenerative diseases, and various types of cancer bound to redox imbalance, inflammation, oxidative stress, and insulin resistance.
Participants
Participant | Institution | |
---|---|---|
Souvenir Rhonda | US CA Los Angeles_Shirihai O, UCLA, US | |
Stiles Linsey | US CA Los Angeles_Shirihai O, UCLA, US | |
Tran Peter | US CA Los Angeles_Shirihai O, UCLA, US | |
Zhang Yuan | US CA Los Angeles_Shirihai O, UCLA, US | |
Zhou Zhenqi | US CA Los Angeles_Shirihai O, UCLA, US |
IOC recommended reading
O2k-Workshops are listed as MitoGlobal Events.'
Labels:
HRR: Oxygraph-2k
ORO, IOC, O2k-Network, 2023, MitoGlobal