MiPNet28.06 IOC161 Coimbra PT: Difference between revisions

Revision as of 13:52, 18 April 2023

MiPNet28.06 IOC161 Coimbra PT

Cantanhede, PT, 2023 Apr 12. Oroboros O2k-Workshop on high-resolution respirometry (HRR), IOC161.

Oroboros (2023-04-12) Mitochondr Physiol Network

Abstract:

Oroboros O2k-Workshop on high-resolution respirometry (HRR) in cooperation with Oliveira Paulo J. Cantanhede, PT, 2023 April 12.

• O2k-Network Lab: AT_Innsbruck_Oroboros

General information

Oroboros Instruments (Innsbruck, Austria) - 161^st O2k-Workshop, Cantanhede

Cutting-edge innovations of high-resolution respirometry (HRR) are presented by Erich Gnaiger, introducing the Oroboros NextGen-O2k [1] in parallel to hands-on training by Sabine Schmitt.

HRR targets reproducibility and flexibility of substrate-uncoupler-inhibitor titration protocols [2] for investigating mitochondrial pathway and coupling control [3]. ROS production, ATP production, mt-membrane potential, and calcium uptake can be measured simultaneously with HRR in each chamber. Modules for monitoring Q- and NADH-redox states and PhotoBiology with the NextGen-O2k open new windows for bioenergetic investigations.

NextGen-O2k all-in-one
SUIT protocols - The SUITbrowser helps you find the best SUIT protocol for your specific research questions.
Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Simultaneous measurement of mitochondrial respiration and hydrogen peroxide production (ROS)

Venue and organizers

UC Biotech Building, Lot 8A, Biocant Park

3060-197 Cantanhede

Portugal

Dr. Oliveira Paulo J, University of Coimbra, PT
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Sabine Schmitt, PhD, Oroboros Instruments - Mitochondrial Detective

IOC recommended reading

» IOC recommended reading

O2k-Workshops are listed as MitoGlobal Events.'

Labels:

HRR: Oxygraph-2k

ORO, IOC, O2k-Network, 2023, MitoGlobal

@@ Line 16: / Line 16: @@
 :::: '''Oroboros Instruments (Innsbruck, Austria) - 161<sup>st</sup> O2k-Workshop, Cantanhede'''
 :::: Cutting-edge innovations of '''high-resolution respirometry (HRR)''' are presented by ''Erich Gnaiger'', introducing the '''Oroboros NextGen-O2k''' [1] in parallel to hands-on training by ''Sabine Schmitt''.
-:::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. ROSproduction, ATP production, mt-membrane potential, and calcium uptake can be measured simultaneously with HRR in each chamber. Modules for monitoring '''Q- and NADH-redox states and PhotoBiology''' with the NextGen-O2k open new windows for bioenergetic investigations.
+:::: HRR targets reproducibility and flexibility of '''substrate-uncoupler-inhibitor titration protocols''' [2] for investigating '''mitochondrial pathway and coupling control''' [3]. ROS production, ATP production, mt-membrane potential, and calcium uptake can be measured simultaneously with HRR in each chamber. Modules for monitoring '''Q- and NADH-redox states and PhotoBiology''' with the NextGen-O2k open new windows for bioenergetic investigations.
 ::::# [https://www.oroboros.at/index.php/product/nextgen-o2k_all-in-one/ NextGen-O2k all-in-one]
 ::::# [[MitoPedia:_SUIT |SUIT protocols]] - The [[SUITbrowser]] helps you find the best SUIT protocol for your specific research questions.
 ::::# Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
+::::# Simultaneous measurement of mitochondrial respiration and [[hydrogen peroxide]] production ([[ROS]])
 == Venue and organizers ==