SUIT-022: Difference between revisions
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{{MitoPedia | {{MitoPedia | ||
|info='''A: | |abbr=AOX (ce CN+SHAM) | ||
|description= [[File:ce1;ce2KCN;ce3SHAM.v2.png|400px]] | |||
|info='''A: Determination of the respiration due to the alternative oxidase pathway''' | |||
}} | }} | ||
::: '''[[SUIT protocol pattern]]:''' ce1;ce2KCN;ce3SHAM | |||
::: '''[[SUIT protocol pattern]]:''' | |||
The SUIT-022 | The SUIT-022 provides an estimation of mitochondrial respiration in cells through the Q-[[alternative oxidase]] pathway (Q-AOX), which is [[Antimycin_A| antimycin A]]- and [[cyanide| cyanide]] resistant. This protocol must be complemented with the study in parallel of mitochondrial respiration due to the CIII-[[Complex_IV| cytochrome ''c'' oxidase]] pathway (CIII-CIV; see [[SUIT-023]]). ย | ||
__TOC__ | __TOC__ | ||
ย Communicated by [[Huete-Ortega M]] | ย Communicated by [[Huete-Ortega M]], [[Gnaiger E]] (last update 2021-03-23) | ||
ย | |||
== Specific SUIT protocols == | == Specific SUIT protocols == | ||
=== SUIT-022 O2 ce | === SUIT-022 O2 ce D051 === | ||
[[File:ce1;ce2KCN;ce3SHAM.png|400px]] | [[File:ce1;ce2KCN;ce3SHAM.v2.png|400px]] [[File:ce1;ce2KCN;ce3SHAM.png|400px]] | ||
[[File: | [[File:D051_O2_traces.png|400px]] | ||
*ย [[SUIT-022 O2 ce | *ย [[SUIT-022 O2 ce D051]] for living microalgal cells (ce) | ||
{{Template:SUIT-022 O2 ce D051}} | |||
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{{Template:SUIT-022}} | |||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of | :::* SUIT-022 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-resistant mitochondrial respiration due to the activity of [[alternative oxidase]] in cells on [[ROUTINE]]. To complement the study with the estimation of mitochondrial respiration due to the CIII-CIV pathway, it must be carried out in parallel to [[SUIT-023]]. | ||
:::+ This protocol can be used with | :::+ This protocol can be used with many types of cells, although optimal concentrations of inhibitors have to be determined. | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
:::- | :::- No estimation of coupling control | ||
:::- The use of [[cyanide| cyanide]] requires | :::- The use of [[cyanide| cyanide]] requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety. | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::*[[SUIT-023 O2 ce D053]] for [[microalgal]] intact cells | |||
== References == | == References == | ||
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|mitopedia method=Respirometry | |mitopedia method=Respirometry | ||
}} | }} | ||
Latest revision as of 12:31, 23 March 2021
Description
Abbreviation: AOX (ce CN+SHAM)
Reference: A: Determination of the respiration due to the alternative oxidase pathway
- SUIT protocol pattern: ce1;ce2KCN;ce3SHAM
The SUIT-022 provides an estimation of mitochondrial respiration in cells through the Q-alternative oxidase pathway (Q-AOX), which is antimycin A- and cyanide resistant. This protocol must be complemented with the study in parallel of mitochondrial respiration due to the CIII- cytochrome c oxidase pathway (CIII-CIV; see SUIT-023).
Communicated by Huete-Ortega M, Gnaiger E (last update 2021-03-23)
Specific SUIT protocols
SUIT-022 O2 ce D051
- SUIT-022 O2 ce D051 for living microalgal cells (ce)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2KCN | ROUTINE or ROX | ce1;ce2KCN
| ||
ce3SHAM | ROX | ce1;ce2KCN;ce3SHAM
|
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- Coupling control
- Pathway control
- ยป Electron transfer pathway
- ยป Fatty acid oxidation pathway control state, F
- ยป NADH electron transfer-pathway state, N
- ยป Succinate pathway control state, S
- ยป NS-pathway control state, NS
- ยป Glycerophosphate pathway control state, Gp
- ยป Complex IV single step, CIV
- ยป Anaplerotic pathway control state
- Pathway control
- Main fuel substrates
- ยป Glutamate, G
- ยป Glycerophosphate, Gp
- ยป Malate, M
- ยป Octanoylcarnitine, Oct
- ยป Pyruvate, P
- ยป Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-022 provides an estimation of the cyanide-and antimycin A-resistant mitochondrial respiration due to the activity of alternative oxidase in cells on ROUTINE. To complement the study with the estimation of mitochondrial respiration due to the CIII-CIV pathway, it must be carried out in parallel to SUIT-023.
- + This protocol can be used with many types of cells, although optimal concentrations of inhibitors have to be determined.
- + Short duration of experiment
- - No estimation of coupling control
- - The use of cyanide requires careful cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-023 O2 ce D053 for microalgal intact cells
References
MitoPedia concepts: MiP concept, SUIT protocol, Recommended
MitoPedia methods:
Respirometry