Difference between revisions of "SUIT-023 O2 ce D053"
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|description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | |description=[[File:ce1;ce2SHAM;ce3KCN.png|350px]] | ||
|info='''A''' Determination of the respiration due to the cytochrome c pathway -'''[[SUIT-023]]''' | |info='''A''' Determination of the respiration due to the cytochrome ''c'' oxidase pathway -'''[[SUIT-023]]''' | ||
|application=O2 | |application=O2 | ||
|SUIT number=D053_ce1;ce2SHAM;ce3KCN | |SUIT number=D053_ce1;ce2SHAM;ce3KCN | ||
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::: '''[[MitoPedia: SUIT]]''' | ::: '''[[MitoPedia: SUIT]]''' | ||
::: '''[[Categories of SUIT protocols |SUIT-category]]:''' | ::: '''[[Categories of SUIT protocols |SUIT-category]]:''' ce | ||
::: '''[[SUIT protocol pattern]]:''' | ::: '''[[SUIT protocol pattern]]:''' linear ce1;ce2SHAM;ce3KCN | ||
The SUIT-023 O2 ce D053 has been designed to provide an estimation of the mitochondrial respiration in [[microalgal]] intact cells due to the cytochrome ''c'' oxidase pathway, which is non-resistant to [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]] inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the [[alternative oxidase]] pathway (see [[SUIT-022]]). | |||
__TOC__ | __TOC__ | ||
Communicated by | Communicated by [[Huete-Ortega M]] and [[Gnaiger E]] (last update 2019-04-09) | ||
{{Template:SUIT-022 O2 ce D00SHAM}} | {{Template:SUIT-022 O2 ce D00SHAM}} | ||
== Strengths and limitations == | == Strengths and limitations == | ||
:::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration | :::* SUIT-023 O2 ce D053 provides an estimation of the [[cyanide| cyanide]]-and [[Antimycin_A| antimycin A]]-non-resistant mitochondrial respiration in [[microalgal]] intact cells. | ||
::: | :::* The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species ''Chlamydomonas reinhardtii'' | ||
:::+ Short duration of experiment | :::+ Short duration of experiment | ||
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== Compare SUIT protocols == | == Compare SUIT protocols == | ||
::::* [[SUIT- | ::::* [[SUIT-022 O2 ce D051]] has to be used in parallel to obtain complete information of the contribution of both [[alternative oxidase]] and cytochrome ''c'' oxidase pathways to mitochondrial respiration. | ||
Revision as of 11:43, 9 April 2019
Description
Abbreviation: ?
Reference: A Determination of the respiration due to the cytochrome c oxidase pathway -SUIT-023
SUIT number: D053_ce1;ce2SHAM;ce3KCN
O2k-Application: O2
- MitoPedia: SUIT
- SUIT-category: ce
- SUIT protocol pattern: linear ce1;ce2SHAM;ce3KCN
The SUIT-023 O2 ce D053 has been designed to provide an estimation of the mitochondrial respiration in microalgal intact cells due to the cytochrome c oxidase pathway, which is non-resistant to cyanide-and antimycin A inhibition. This protocol must be complemented with the study in parallel of the mitochondrial respiration due to the alternative oxidase pathway (see SUIT-022).
Communicated by Huete-Ortega M and Gnaiger E (last update 2019-04-09)
Steps and respiratory states
Step | State | Pathway | Q-junction | Comment - Events (E) and Marks (M) |
---|---|---|---|---|
ce1 | ROUTINE | ce1
| ||
ce2SHAM | ROX | ce1;ce2SHAM
| ||
ce3KCN | ROX | ce1;ce2SHAM;ce3KCN
|
- Bioblast links: SUIT protocols - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Coupling control
- Pathway control
- Main fuel substrates
- » Glutamate, G
- » Glycerophosphate, Gp
- » Malate, M
- » Octanoylcarnitine, Oct
- » Pyruvate, P
- » Succinate, S
- Main fuel substrates
- Glossary
Strengths and limitations
- SUIT-023 O2 ce D053 provides an estimation of the cyanide-and antimycin A-non-resistant mitochondrial respiration in microalgal intact cells.
- The concentrations of inhibitors used in this protocol have been optimised for the specific case of the microalgal model species Chlamydomonas reinhardtii
- + Short duration of experiment
- - Estimation of the linear coupling control is not done
- - The use of cyanide requires a careful and in deep cleaning of the chambers after use and careful handling of the chemical to guarantee health safety.
Compare SUIT protocols
- SUIT-022 O2 ce D051 has to be used in parallel to obtain complete information of the contribution of both alternative oxidase and cytochrome c oxidase pathways to mitochondrial respiration.
References
MitoPedia concepts: SUIT protocol, SUIT A, Find
MitoPedia methods:
Respirometry