Test - Ecosystem agenda

Test - Ecosystem agenda

Bioblast alert - 2020

Bioblast alert 2020(18) - Bioenerg Commun & MitoFit Preprints: 2020-12-07

Adressing the importance for biotechnological exploitations

Methodology enabling expansion of current knowledge in the field of chloroplast and algal bioenergetics:

Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. MitoFit Preprint Arch doi:10.26124/mitofit:ea20.algaeurope.0001. - »Bioblast link«

Submit your preprint to MitoFit Preprints and increase awareness of your research::

Journal articles that were uploaded as preprints before being published gather more citations in the long run than papers without a preprint version. The effect is immediate and long-lasting.

» How to submit your preprint«

To publish scientific and technical advances in bioenergetics and mitochondrial physiology in living communications, submit your paper to the Open Access journal Bioenergetics Communications.

» Bioenergetics Communications «

Bioblast alert 2020(17) - H₂O₂ flux: 2020-11-30

Background H₂O₂ calibration in the Amplex® UltraRed (AmR) assay:

A background H₂O₂ calibration is performed before addition of sample. This is required for (1) calculation of the chemical background fluorescence in our Excel analysis template, and (2) calculation AmR assay sensitivity, for quality control.
- »Background H₂O₂ calibration«

Why do we perform multiple H₂O₂ calibrations in the Amplex® UltraRed assay?

The sensitivity to H₂O₂ of the fluorometric signal changes during the experiment as a function of: (1) experimental time and accumulating UltroxRed (similar to resorufin), (2) changes of the optical properties due to titrations, and (3) the radical scavenging capacity of the sample. Therefore, H₂O₂ calibrations are performed before and after sample addition, and after selected titration steps.

- »Amplex UltraRed« and »Komlodi 2018 Methods Mol Biol«

Are permeabilized muscle fibers (pfi) a reliable model for studying ROS production?

High oxygen concentrations are needed to avoid oxygen limitation in pfi. However, high oxygen pressures may artificially increase ROS prodcution, including H₂O₂, making pfi a questionable model for ROS production.
- »Oxygen dependence of ROS production in pfi«

Bioblast alert 2020(16) - PBMC and Platelets: 2020-10-23

Childhood maltreatment is associated with increased mitochondrial bioenergetic function of immune cells in mothers, but not in their newborns

from the O2k-Network »DE Ulm Gumpp A«

Gumpp AM, Boeck C, Behnke A, Bach AM, Ramo-Fernández L, Welz T, Gündel H, Kolassa IT, Karabatsiakis A (2020) Childhood maltreatment is associated with changes in mitochondrial bioenergetics in maternal, but not in neonatal immune cells. Proc Natl Acad Sci U S A 117:24778-84. »Bioblast link«

Human PBMC and Platelets - samples for high-resolution respirometry obtained by minimally invasive procedures.

We compared isolation procedures for blood cells.
- »MiPNet21.17 Blood Cells Isolation«

Explore mitochondrial pathways inPBMC and platelets

The following SUIT protocols are specifically optimized for PBMC and platelets.
- »SUIT-001 O2 ce-pce D004« and »SUIT-002 O2 ce-pce D007a«

Bioblast alert 2020(15) - Upcoming Virtual Events: 2020-09-17

Free registration: European Society for Clinical Investigation Virtual Meeting 2020 – COVID19 edition - Sep 20-30, 2020

- »ESCI 2020 Virtual Event«

Registration open: 12th ÖGMBT Annual Meeting - Biomolecules in/for 21st century

Kick-off Sep 21-23, 2020
Life Science Tuesdays Oct 2020 - Mar 2021

- »12th ÖGMBT Annual Meeting 2020 Virtual Event«

Public event: Long Night of Research (some content in German) - Oct 9, 2020

- »Long Night of Research 2020 Virtual Event«

Bioblast alert 2020(14) - The potential of PBMCs in HRR: 2020-09-08

Mitochondrial respiration in PBMCs from patients with major depression

from the O2k-Network »DE Ulm Karabatsiakis A« and »DE Ulm Radermacher P«

Karabatsiakis A, Boeck C, Salinas-Manrique J, Kolassa S, Calzia E, Dietrich DE, Kolassa IT (2014) Mitochondrial respiration in peripheral blood mononuclear cells correlates with depressive subsymptoms and severity of major depression. Transl Psychiatry 4:e397. »Bioblast link«

Mitochondrial respiration in PBMCs: a potential parameter to monitor acute pancreatitis

from the O2k-Network »NZ Auckland Hickey AJ«

Chakraborty M, Hickey AJ, Petrov MS, Macdonald JR, Thompson N, Newby L, Sim D, Windsor JA, Phillips AR (2016) Mitochondrial dysfunction in peripheral blood mononuclear cells in early experimental and clinical acute pancreatitis. Pancreatology 16:739-47. »Bioblast link«

Low-intensity exercise increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults

from the O2k-Network »LV Riga Makrecka-Kuka M«

Liepinsh E, Makarova E, Plakane L, Konrade I, Liepins K, Videja M, Sevostjanovs E, Grinberga S, Makrecka-Kuka M, Dambrova M (2020) Low-intensity exercise stimulates bioenergetics and increases fat oxidation in mitochondria of blood mononuclear cells from sedentary adults. Physiol Rep 8:e14489. »Bioblast link«

Bioblast alert 2020(13) - MitoFit Preprints & BEC news: 2020-08-12

Some terms in “Mitochondrial physiology” Bioenergetics Communications 2020.1 (States and rates) did not look familiar ― what is the elementary unit [x]?

Fundamental answers, a critical discussion on the SI unit for count, and the meaning of Euclid’s unit and Euclid’s number are presented in an unusual ‘canonical review’:

Gnaiger Erich (2020) Canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. MitoFit Preprint Arch 2020.4 doi:10.26124/mitofit:200004. - »Bioblast link«

Join the discussion on the MitoEAGLE Consortium Communication: “Mitochondrial physiology” Bioenergetics Communications 2020.1

» On a Comment in Nature Metabolism «

Bioblast alert 2020(12) - O2k-Publications: 2020-07-17

Alternative oxidase inhibition plays a key role in the aluminum tolerance of cultured Jatropha curcas cells

from the O2k-Network »BR Ribeirao Preto Alberici LC«

Vicentini TM, Cavalheiro AH, Dechandt CRP, Alberici LC, Vargas-Rechia CG (2019) Aluminum directly inhibits alternative oxidase pathway and changes metabolic and redox parameters on Jatropha curcas cell culture. Plant Physiol Biochem 136:92-97. »Bioblast link«

Mitochondrial H₂O₂ flux in cardiac and skeletal muscle preparations is influenced by experimental oxygen concentration

from the O2k-Network »US CO Fort Collins Chicco AJ«

Li Puma LC, Hedges M, Heckman JM, Mathias AB, Engstrom MR, Brown AB, Chicco AJ (2020) Experimental oxygen concentration influences rates of mitochondrial hydrogen peroxide release from cardiac and skeletal muscle preparations. Am J Physiol Regul Integr Comp Physiol 318:R972-80. »Bioblast link«

Influenza A infection induces persistent alterations in whole-body glucose metabolism in mice

from the O2k-Network »FR Lille Duez H«

Ayari A, Rosa-Calatrava M, Lancel S, Barthelemy J, Pizzorno A, Mayeuf-Louchart A, Baron M, Hot D, Deruyter L, Soulard D, Julien T, Faveeuw C, Molendi-Coste O, Dombrowicz D, Sedano L, Sencio V, Le Goffic R, Trottein F, Wolowczuk I (2020) Influenza infection rewires energy metabolism and induces browning features in adipose cells and tissues. Commun Biol 3:237. »Bioblast link«

The antidiabetic drug empagliflozin protects cardiac mitochondria against high-fat diet-induced dysfunction in mice

from the O2k-Network »LV Riga Makrecka-Kuka M«

Makrecka-Kuka M, Korzh S, Videja M, Vilks K, Cirule H, Kuka J, Dambrova M, Liepinsh E (2020) Empagliflozin protects cardiac mitochondrial fatty acid metabolism in a mouse model of diet-induced lipid overload. Cardiovasc Drugs Ther [Epub ahead of print]. »Bioblast link«

Bioblast alert 2020(11) - O2k-Publications: 2020-05-20

F₁F_O-ATP synthase regulates alternative oxidase in Trypanosoma brucei

from the O2k-Network »BR Sao Paulo Silber AM«

Luévano-Martínez LA, Girard RMBM, Alencar MB, Silber AM (2020) ATP regulates the activity of an alternative oxidase in Trypanosoma brucei. FEBS Lett [Epub ahead of print]. »Bioblast link«

Restoration of mitochondrial oxygen consumption and decrease of mitochondrial ROS by AOX in the post-ischaemic heart are not sufficient to confer acute or chronic cardioprotection in mice

from the O2k-Network » FI Helsinki Jacobs HT« , »DE Magdeburg Gellerich FN« , »FI Tampere Dufour E« , »DE Magdeburg Debska-Vielhaber G« and »DE Frankfurt Wittig I«

Szibor M, Schreckenberg R, Gizatullina Z, Dufour E, Wiesnet M, Dhandapani PK, Debska-Vielhaber G, Heidler J, Wittig I, Nyman TA, Gaertner U, Hall AR, Pell V, Viscomi C, Krieg T, Murphy MP, Braun T, Gellerich Frank N, Schlueter KD, Jacobs HT(2020) Respiratory chain signalling is essential for adaptive remodelling following cardiac ischaemia. J Cell Mol Med 24:3534-48.. - »Bioblast link«

Physical activity has positive effects on mitochondria from patients with occupational burnout

from the O2k-Network »CH Basel Eckert A«

Brand S, Ebner K, Mikoteit T, Lejri I, Gerber M, Beck J, Holsboer-Trachsler E, Eckert A (2020) Influence of regular physical activity on mitochondrial activity and symptoms of burnout - an interventional pilot study. J Clin Med 9:E667. - »Bioblast link«

Allergic rhinitis induces mitochondrial dysfunction in peripheral blood cells«

from the O2k-Network »FR Strasbourg Zoll J«

Qi S, Barnig C, Charles AL, Poirot A, Meyer A, Clere-Jehl R, de Blay F and Geny Bernard
(2017) Nasal allergen challenge in allergic rhinitis induces mitochondrial dysfunction of peripheral blood mononuclear cells. Ann Allergy Asthma Immunol 118:367-9. »Bioblast link«

Bioblast alert 2020(10) - MitoEAGLE & BEC news: 2020-05-20

MitoEAGLE Consortium Communication: Mitochondrial physiology ― Published online: 2020-05-20.

Gnaiger E et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1
» https://www.bioenergetics-communications.org/index.php/BEC2020.1_doi10.26124bec2020-0001.v1

SI units

One year ago, on World Metrology Day (2019-May-20) the redefinition of the SI units came into force. Science faced one of the largest overhauls in history of scientific units. How to commemorate this event of groundbreaking innovation better than with the online publication of Mitochondrial physiology and with the launch of an innovative journal — Bioenergetics Communications.

Bioblast alert 2020(09) - MitoEAGLE News: 2020-04-09

The MitoEAGLE whitepaper on ‘Mitochondrial respiratory states and rates’ is now ready for re-submission to Nature Metabolism. The full-length preprint with a total word count of 17,000 has been reduced to 3,000 words text plus figures and references, including all original sections.

Version 6 of the paper with all 620 coauthors is available here:

» www.mitoeagle.org/index.php/Gnaiger_2019_MitoFit_Preprint_Arch

Please contact us, if you are interested to see the shortened version of the manuscript.
If you intend to join as an additional coauthor, please send your comments, full institutional address and preferentially your ORCID iD to [email protected]

The July MitoEAGLE Summit and Training School (including the TRANSMIT workshop) have been postponed by the management committee. More information to follow.

» https://wiki.oroboros.at/index.php/MitoEAGLE_Summit_Obergurgl_2020

» https://wiki.oroboros.at/index.php/MiPschool_Obergurgl_2020

Bioblast alert 2020(08) - MitoFit Preprints News: 2020-03-25

Vitamin D deficiency: a factor in COVID-19 progression severity and mortality? An urgent call for research

Brown RA, Sarkar A (2020) Vitamin D deficiency: a factor in COVID-19, progression, severity and mortality? – An urgent call for research. MitoFit Preprint Arch doi:10.26124/mitofit:200001.

Bioblast alert 2020(07): 2020-03-20

From MitoFit Preprints to Nature Communications

High-resolution respirometry in human prostate cancer biopsies and mtDNA analyzed in samples recovered from the O2k-chamber

- from the O2k-Network »AT Innsbruck Oroboros« Schöpf Bernd, Weissensteiner Hansi, Schäfer Georg, Fazzini Federica, Charoentong Pornpimol, Naschberger Andreas, Rupp Bernhard, Fendt Liane, Bukur Valesca, Giese Irina, Sorn Patrick, Sant’Anna-Silva Ana Carolina, Iglesias-Gonzalez Javier, Sahin Ugur, Kronenberg Florian, Gnaiger Erich, Klocker Helmut (2020) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and increased succinate oxidation. Nat Commun 2020-03-20 [Epub ahead of print]. - »Bioblast link«

OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature

Schöpf et al (2019) OXPHOS remodeling in high-grade prostate cancer involves mtDNA mutations and a prognostic gene expression signature. MitoFit Preprint Arch doi:10.26124/mitofit:190003. - »Bioblast link«

Do you know what a preprint is? With a preprint you can communicate recent findings to the scientific community, receiving input from other scientists before submitting to a peer-reviewed journal. - »Preprint«

Bioblast alert 2020(06): 2020-02-26

Research is needed on the impact of viral infection on mitochondrial function.

- »Viruses and mitochondrial medicine«

High quality mitochondrial research is increasing rapidly in South Korea.

- »O2k-Network Labs Republic of Korea«

An active lifestyle protects cardiac mitochondria

- from the O2k-Network: »KR Busan Han J«, »KR Incheon Kwak HB«

No MH, Heo JW, Yoo SZ, Kim CJ, Park DH, Kang JH, Seo DY, Han J, Kwak HB (2020) Effects of aging and exercise training on mitochondrial function and apoptosis in the rat heart. Pflugers Arch 472:179-93. - »Bioblast link«

It is essential to titrate uncouplers in multiple steps for respirometric measurement of ET capacity.

- »Noncoupled respiration«

Bioblast alert 2020(05): 2020-02-20

Registration now open! 21st European Bioenergetics Conference. Marseille, France. 2020 Aug 22-27.

- »EBEC2020«

Aging and hyperlipidemia impair aortic mitochondrial function and increase the risk of atherosclerosis

- from the O2k-Network US MI Ann Arbor Beard DA Tyrrell DJ, Blin M, Song J, Wood S, Zhang M, Beard DA, Goldstein D (2019) Age-associated mitochondrial dysfunction accelerates atherogenesis. Circ Res 126:298–314. »Bioblast link«

Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function.

- »Spermidine«

MitoFit Preprints was initiated one year ago on February 19, 2020.

- We invite submissions of mitochondrial preprints.

Bioblast alert 2020(04): 2020-02-07

Save the date! April 24-25, 2020 - The first meeting of the Bioenergetics DE-CH-AT Group aims at exchange and collaboration in bioenergetics between Germany, Switzerland and Austria.

- »Bioenergetics Vienna 2020«

Blood cell mitochondrial function does not provide a general substitute for muscle biopsies

- from the O2k-Network: US AR Little Rock Boersheim E Rose S, Carvalho E, Diaz EC, Cotter M, Bennuri SC, Azhar G, Frye RE, Adams SH, Boersheim E (2019) A comparative study of mitochondrial respiration in circulating blood cells and skeletal muscle fibers in women. Am J Physiol Endocrinol Metab 317:E503-E512. - »Bioblast link«

Cronin (2018) The metabolite BH4 controls T cell proliferation in autoimmunity and cancer.- »Spermidine«

Nature - Lymphocytes, Cancer - O2k-brief repository

Aerobic and resistance training address central defects and peripheral comorbidities in Alzheimer’s disease

- from the O2k-Network: US FL Boca Raton Khamoui AV

Pena GS, Paez HG, Johnson TK, Halle JL, Carzoli JP, Visavadiya NP, Zourdos MC, Whitehurst MA, Khamoui AV (2020) Hippocampal growth factor and myokine cathepsin B expression following aerobic and resistance training in 3xTg-AD mice. Int J Chronic Diseases 2020:Article ID 5919501. - »Bioblast link«

Bioblast alert 2020(03): 2020-01-31

The European Society for Surgical Research Congress early bird registration deadline is Monday February 10, 2020.

- »ESSR 2020 Innsbruck AT«

Mitochondrial respiration is oxygen-dependent in human cardiac homogenate preparations

- from the O2k-Network: CZ Prague Krajcova A

Krajčová A, Urban T, Megvinet D, Waldauf P, Balík M, Hlavička J, Budera P, Janoušek L, Pokorná E, Duška F (2020) High resolution respirometry to assess function of mitochondria in native homogenates of human heart muscle. PLOS ONE 15:e0226142. - »Bioblast link«

Mitochondrial respiration of blood cells and cardiovascular disease

- from the O2k-Network: FR Strasbourg Zoll J Alfatni A, Riou M, Charles AL, Meyer A, Barnig C, Andres E, Lejay A, Talha S, Geny B (2020) Peripheral blood mononuclear cells and platelets mitochondrial dysfunction, oxidative stress, and circulating mtDNA in cardiovascular diseases. J Clin Med 9 pii:E311. - »Bioblast link«

Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration

In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »Oxygen calibration - DatLab«

Bioblast alert 2020(02): 2020-01-24

The Association for Psychological Science 2020 conference abstract submission deadline for poster presentations is Friday January 31, 2020.

- » Bioblast link«

Dias et al (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem

- Nervous system »Bioblast link«

HRR - high-resolution respirometry and oxygen kinetics 10 years ago: Cell respiration under hypoxia: facts and artefacts in mitochondrial oxygen kinetics. Adv Exp Med Biol 2010.

- »Bioblast link«

Two websites:

- One new: the Oroboros MarketPlace website »www.oroboros.at« - One continued: the website of the Oroboros Ecosystem »www.bioblast.at«

Bioblast alert 2020(01): 2020-01-17

The Oroboros Ecosystem Agenda 2020 is launched as a signature of the Oroboros Ecosystem.

2020: a critical year for women, gender equity, and health - a Lancet Editorial

Statistical evaluation on replica in HRR with permeabilized skeletal muscle fibers

- from the O2k-Network: AU Melbourne Stepto NK Jacques M, Kuang J, Bishop DJ, Yan X, Alvarez-Romero J, Munson F, Garnham A, Papadimitriou I, Voisin S, Eynon N (2019) Mitochondrial respiration variability and simulations in human skeletal muscle: The Gene SMART study. FASEB J [Epub ahead of print]. - »Bioblast link«

Planning the MitoEAGLE Summit Obergurgl 2020

Members of the Management Committee MitoEAGLE and Working Group Leaders: please submit your suggestions for MitoEAGLE Summit Obergurgl 2020: - Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output - Suggested type and title of your personal presentation, according to the MoU and final MitoEAGLE output