MiPNet25.06 IOC145 Innsbruck AT
Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145. |
Oroboros (2020-01-20) Mitochondr Physiol Network
Abstract:
O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 27-29.
β’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
General information
- O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.
Venue
- Β» Oroboros O2k-Laboratory
- Schoepfstrasse 18, 6020 Innsbruck
- Β» Information on travel and venue: IOC Innsbruck
- Β» Oroboros O2k-Laboratory
Registration
Download registration form, fill in and sign the pdf: download pdf
Tutors and lecturers
Luiza Cardoso, PhD., Oroboros Instruments - PostDoc
Marco Di Marcello, BSc., Oroboros Instruments - Research Assistant
Carolina Doerrier, PhD. - Chief scientific officer
Luiz Felipe Garcia-Souza, PhD. student, Oroboros Instruments
Erich Gnaiger, PhD., Oroboros Instruments, CEO
Preliminary programme
Monday 27th
Topic Room 08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros O2k-Laboratory 08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros O2k-Laboratory 09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros O2k-Laboratory 09:55 DatLab 7.4 overview Oroboros O2k-Laboratory 10:30 Coffee 11:00 Instrumental quality control 1: oxygen calibration Oroboros O2k-Laboratory 11:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation) DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 13:30 Instrumental quality control 2: Instrumental O2 background β overview with videoclips Oroboros O2k-Laboratory 14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background DL-Protocol: Instrumental O2 background TiP2k
Oroboros O2k-Laboratory 15:00 Coffee 15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros O2k-Laboratory
Tuesday 28th
Topic Room 08:30 Hands-on: Instrumental quality control 1: oxygen calibration DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols β fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros O2k Laboratory 09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros O2k Laboratory 10:00 Coffee 10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002). DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros O2k Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 13:30 DatLab 7.4 analysis: Introduction and new features Oroboros O2k-Laboratory 15:00 Coffee 15:30-17:30 Individual DatLab analysis + summary Oroboros O2k-Laboratory
Wednesday 29th
Topic Room 08:30 MitoFit proficiency test Hands-on: Instrumental quality control 1: oxygen calibration
DL-Protocol: O2k-cleaning BeforeUse
DL-Protocol: O2 calibration air
Oroboros O2k-Laboratory 10:00 Coffee 10:30 MitoFit proficiency test Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).
DL-Protocol: SUIT-001 O2 ce-pce D003.DLP
DL-Protocol: SUIT-002 O2 ce-pce D007.DLP
Oroboros O2k-Laboratory 11:45 Hands-on: O2k-cleaning after use DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 12:00 Lunch break 13:00 Hands-on: O2k-cleaning after use (continuation) DL-Protocol: O2k-cleaning AfterUse
Oroboros O2k-Laboratory 13:15 DatLab analysis Oroboros O2k-Laboratory 14:00 O2k-Applications - overview Oroboros O2k-Laboratory 14:30 Proficiency test: DatLab analysis summary Oroboros O2k-Laboratory 15:00 Coffee 15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt 16:30 Feedback & conclusions MiPArt 17:00 Farewell activity MiPArt
Participants
Participant | Institution | |
---|---|---|
Oefele Marlene | DE Seewiesen Casagrande - Max Planck Institute for Ornithology, Seewiesen (DE) |
Contact
- Oroboros Instruments GmbH
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- E-Mail: [email protected]
- Mitochondria and Cell Research
IOC recommended reading
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoGlobal, Next, 2020