High signal at zero oxygen |
MitoPedia O2k and high-resolution respirometry:
O2k-Open Support
Description
A high signal at zero oxygen may be observed during zero calibration (R0). First, check the quality of the dithionite solution. The following instructions show how to distinguish between a defective sensor head and an electrical leak current.
Reference: Zero calibration, OroboPOS-Connector
Leak current test
O2k-series J and XB
- Unplug the SmartPOS from the O2k-main unit.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Remove the membrane from the SmartPOS and wash the cathode and anode with distilled water.
- Connect the SmartPOS to the O2k-main unit. Set an event in DatLab called "SmartPOS".
- Record the signal for 10 min.
- Send us the file.
O2k-series D-I and XA
- Unscrew the POS from the POS-Connector and unplug the POS-Connector from the O2k-main unit.
- Switch on the O2k and start DatLab.
- Record the signal for 10 min.
- Connect the POS-Connector to the O2k-main unit without the POS. Set an event in DatLab called "POS-Connector".
- Record the signal for 10 min.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- Send us the file.
O2k-series A-C
- Remove the POS but keep the POS-Connector connected to the main unit. Plugging or unplugging of the POS-Connector while the main unit is switched on could damage the POS-Connector of O2k-series A-C.
- Record the signal for 10 min.
- Remove the membrane from the POS and wash the cathode and anode with distilled water.
- Screw the POS to the POS-Connector. Set an event in DatLab called "POS".
- Record the signal for 10 min.
- Send us the file.
References
Example
- First, the OroboPOS-Connector and the POS were disassembled and disconnected from the main unit. Second, the OroboPOS-Connector was connected to the O2k-Main unit without the OroboPOS. Third, the OroboPOS was connected to the OroboPOS-Connector without membranes. After each step, the O2 raw signal [V] was recorded for 10 min. The marks were set to the O2 raw signal [V] to evaluate the zero current.
- Figure 1. High zero current test. Upper panel: Zero current is in the range of ±0.00099 V. Lower panel: Zero current is higher than 0.00099 V when the OroboPOS-Connector is connected to the O2k-Main unit and when the OroboPOS is connected to the OroboPOS-Connector, indicating a potential electrical problem with both the OroboPOS-Connector and the OroboPOS.
Keywords
- Bioblast links: Oxygen signal - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
- Specific
- O2k-Procedures
- MiPNet O2k-Procedures
- General
- » Oxygen, dioxygen, O2
- » Oxygen calibration - DatLab
- » Oxygen solubility
- » Oxygen solubility factor
- » Oxygen pressure
- » Concentration
- » Activity
- » Pressure - Pascal
- » Barometric pressure
- » High-resolution respirometry
- » OroboPOS
- » Polarographic oxygen sensor
- » MitoFit Quality Control System
- » Sensitivity
- General
- Other keyword lists
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