MiPNet18.06 Amplex-Yeast: Difference between revisions

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MiPNet18.06 Amplex-Yeast

O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. » Update: Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. MitoFit Preprints 2021.10. doi:10.26124/mitofit:2021-0010

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Oroboros (2019-06-24) Mitochondr Physiol Network

Abstract: Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2019) O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(06):1-4.

Open the pdf document above.

» Current O2k-series: NextGen-O2k Series XB and O2k Series J

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Cited by

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HRR: O2k-Protocol

O2k-Demo, H2O2

Bioblast links: Hydrogen peroxide - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Specific

» Amplex UltraRed

» Discussion:Amplex UltraRed

» Horseradish peroxidase

» Hydrogen peroxide

» Resorufin

General

» Extrinsic fluorophores

» MiR05-Kit

» ROS

» Sensitivity

O2k-Procedures

» Komlodi 2021 MitoFit AmR-O2 — AmplexRed, O₂ dependence, yeast

» MiPNet20.14 AmplexRed H2O2-production

» MiPNet18.05 Amplex-Mouse-heart

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+{{OROBOROS header page name}}
 {{Publication
-|title=Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2013) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06.
+|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast.
-|info=[[Media:MiPNet18.06 Amplex-Yeast.pdf|'''Open Access-MiPNet18.06.pdf''']]; [http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions]
-[[Image:O2k-Protocols.jpg|right|150px|link=http://www.oroboros.at/?o2k-protocols|O2k-Protocols contents]]
-|authors=OROBOROS
+» ''Update:'' Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. MitoFit Preprints 2021.10. [[doi:10.26124/mitofit:2021-0010]]
-|year=2013
+|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/f/f9/MiPNet18.06_Amplex-Yeast.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet18.06_Amplex-Yeast.pdf Versions]
+|authors=Oroboros
+|year=2019-06-24
 |journal=Mitochondr Physiol Network
-|abstract=
+|abstract=Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2019) O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06(06):1-4.
+{{MiPNet pdf page linking to MitoPedia}}
+:» Product: [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
+|mipnetlab=AT Innsbruck Oroboros, AT Innsbruck MitoCom
+}}
+== Cited by ==
-Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the [[O2k-Fluorescence LED2-Module]]. Extracellular H<sub>2</sub>O<sub>2</sub>-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H<sub>2</sub>O<sub>2</sub> production (Fig. 2).
-:>> O2k-Protocols:[http://www.oroboros.at/?o2k-protocols Overall contents]
-:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS Oxygraph-2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
-|titel=O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.
-|link=
-|editor=
-}}
 {{Labeling
-|area=Respiration
+|instruments=O2k-Protocol
-|organism=Saccharomyces cerevisiae
+|additional= O2k-Demo, H2O2
-|taxonomic group=Fungi
-|preparations=Intact cells
-|topics=O2, Uncoupler
-|couplingstates=ROUTINE, ETS
-|instruments=Oxygraph-2k, Fluorometry, Protocol
-|additional=Label
-|titel=O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast.
-|link=
-|editor=
 }}
-== Further information ==
+{{Keywords: H2O2}}
-:>> [http://oroboros.at/?H2O2-yeast O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker's yeast]