User talk:Tindle Lisa/My sandbox O2k-Procedures |
O2 consumption
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Gnaiger 2020 BEC MitoPathways | published online 2020-12-30
| Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002 | Permeabilized cells Permeabilized tissue Homogenate Isolated mitochondria Intact cells | Heart Skeletal muscle Fibroblast | Human Mouse | 2020 |
MiPNet12.01 Suppl T-issue | »Versions | MitoPathways at the Q-junction: mouse skeletal muscle fibers. | Permeabilized tissue | Skeletal muscle | Mouse | 2018-11-18 |
MiPNet21.14 Reference sample HRR | »Versions | Development of a reference sample for HRR. | Intact cells Permeabilized cells | HEK | Human | 2016-10-19 |
MiPNet08.09 CellRespiration | »Versions | High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX. | Intact cells | Blood cells Lymphocyte | Human | 2016-08-11 |
MiPNet17.03 Shredder vs Fibers | »Versions | Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder. | Homogenate | Heart Liver | Fishes | 2015-06-18 |
MiPNet12.11 MitoRespiration | »Versions | Flux control ratios in isolated mitochondria. OXPHOS capacity and respiratory control. | Isolated mitochondria | Artemia Crustaceans | 2014-02-20 | |
Pesta 2012 Methods Mol Biol | PMID: 22057559 | Pesta D, Gnaiger E (2012) High-resolution respirometry. OXPHOS protocols for human cells and permeabilized fibers from small biopsies of human muscle. Methods Mol Biol 810:25-58. https://doi.org/10.1007/978-1-61779-382-0_3 | Permeabilized cells Permeabilized tissue Intact cells | Skeletal muscle Other cell lines HEK Fibroblast HUVEC | Human | 2012 |
MiPNet08.12 IOC22 | NO effect on mitochondrial oxygen kinetics at low oxygen. O2k workshop Report. | Isolated mitochondria | Liver | Rat | 2011-12-11 | |
Gnaiger 2008 POS | Gnaiger E (2008) Polarographic oxygen sensors, the oxygraph and high-resolution respirometry to assess mitochondrial function. In: Mitochondrial dysfunction in drug-induced toxicity (Dykens JA, Will Y, eds) John Wiley & Sons, Inc, Hoboken, NJ:327-52. | Permeabilized cells Permeabilized tissue Intact cells | Blood cells | Mouse | 2008 | |
MiPNet04.05 Titration-Injection | From step titration to ramp injection: Uncoupling by FCCP with TIP. | Intact cells | Endothelial;epithelial;mesothelial cell HUVEC | Human | 1999-01 |
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Redox biology
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Komlodi 2021 BEC Q | Bioenerg Commun 2021.3. published online 2021-11-11
| Komlódi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003 | Isolated mitochondria | Heart Nervous system | Mouse | 2021 |
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H2O2 production
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Komlodi 2021 BEC AmR-O2 | Bioenerg Commun 2021.4. published online 2021-12-21
| Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004 | Intact cells | Other cell lines | Saccharomyces cerevisiae | 2021 |
MiPNet18.06 Amplex-Yeast | »Versions | O2k-FluoRespirometry: Amplex UltraRed using freeze-dried baker’s yeast. » Update: Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. MitoFit Preprints 2021.10. doi:10.26124/mitofit:2021-0010 | 2019-06-24 | |||
MiPNet20.14 AmplexRed H2O2-production | »Versions | O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed. | Isolated mitochondria | Heart | Mouse | 2019-06-24 |
Komlodi 2018 Methods Mol Biol | PMID:29850998 | Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. | Permeabilized cells Permeabilized tissue | Skeletal muscle HEK | Human Mouse | 2018 |
Makrecka-Kuka 2015 Biomolecules | Biomolecules 5:1319-38. PMID: 26131977 Open Access | Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319 | Permeabilized cells Homogenate Isolated mitochondria Intact cells | Heart Nervous system HEK | Human Mouse | 2015 |
Krumschnabel 2015 Methods Mol Biol | PMID: 25631019 | Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. | Isolated mitochondria | Nervous system | Mouse | 2015 |
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mt-Membrane potential
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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MiPNet20.13 Safranin mt-membranepotential | »Versions | O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM. | Homogenate | Nervous system | Mouse | 2019-06-24 |
MiPNet14.05 TPP-mtMembranePotential | »Versions | TPP+ and mt-membrane potential. | 2016-04-01 | |||
Krumschnabel 2014 Methods Enzymol | PMID: 24862266 » | Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. Methods Enzymol 542:163-81. https://doi.org/10.1016/B978-0-12-416618-9.00009-1 | Homogenate | Nervous system | Mouse | 2014 |
MiPNet15.08 TPP electrode | »Versions | Ion selective electrode for TPP+ and high-resolution respirometry. | 2011-12-11 |
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ATP production
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Cardoso 2021 BEC MgG | Bioenerg Commun 2021.1. published online 2021-06-30 | Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001 | Isolated mitochondria | Heart | Mouse | 2021 |
Chinopoulos 2014 Methods Enzymol | PMID: 24862274 Open Access» | Chinopoulos C, Kiss G, Kawamata H, Starkov AA (2014) Measurement of ADP-ATP exchange in relation to mitochondrial transmembrane potential and oxygen consumption. Methods Enzymol 542:333-48. doi:10.1016/B978-0-12-416618-9.00017-0 | Permeabilized cells | HEK | Human | 2014 |
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Calcium
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Naszai 2019 Sci Rep | PMID: 31848391 Open Access | Nászai A, Terhes E, Kaszaki J, Boros M, Juhász L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229. | Isolated mitochondria | Liver | Rat | 2019 |
Elustondo 2014 Biochim Biophys Acta | PMID: 25448536 | Elustondo PA, Negoda A, Kane CL, Kane DA, Pavlov EV (2014) Spermine selectively inhibits high-conductance, but not low-conductance calcium-induced permeability transition pore. Biochim Biophys Acta 1847:231–40. | Isolated mitochondria | Liver | Rat | 2014 |
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Plant physiology
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Went 2021 MitoFit PB | MitoFit Preprints 2021.05. | Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005 | Intact cells | Algae | 2021 |
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NO concentration
Protocol | View | Topic | Preparation | Tissue and cell | Mammal and model | Last update |
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Aguirre 2010 Biochim Biophys Acta | PMID: 20144583 Open Access | Aguirre E, Rodríguez-Juárez F, Bellelli A, Gnaiger E, Cadenas S (2010) Kinetic model of the inhibition of respiration by endogenous nitric oxide in intact cells. Biochim Biophys Acta 1797:557-65. https://doi.org/10.1016/j.bbabio.2010.01.033 | Intact cells Enzyme Oxidase;biochemical oxidation | HEK | 2010 |
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O2k-Procedures | |
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